Pseudomonas aeruginosa UCBPP-PA14, PA14_62000 (hitA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006)
GCF_000014625.1|latest
Locus Tag
PA14_62000
Name
hitA
Replicon chromosome
Genomic location 5529629 - 5530636 (+ strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 5 transposon mutants in orthologs

Cross-References

RefSeq YP_793149.1
GI 116052832
Entrez 4384625
INSDC ABJ14068.1
NCBI Locus Tag PA14_62000
UniParc UPI00000C5DC7
UniProtKB Acc Q02FY7
UniProtKB ID Q02FY7_PSEAB
UniRef100 UniRef100_W0YXB5
UniRef50 UniRef50_V6JBE1
UniRef90 UniRef90_A6VCD9

Product

Feature Type CDS
Coding Frame 1
Product Name
ferric iron-binding periplasmic protein HitA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -4.82
Kyte-Doolittle Hydrophobicity Value -0.223
Molecular Weight (kDa) 36.1
Isoelectric Point (pI) 5.48

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6IVY STRUCTURAL PROTEIN 12/04/18 Crystal structure of iron-bound HitA from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 2 X-RAY DIFFRACTION 100.0
6IWF METAL BINDING PROTEIN 12/05/18 Crystal structure of HitA from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 1.70662 X-RAY DIFFRACTION 100.0
6J2S METAL BINDING PROTEIN 01/02/19 Structure of HitA bound to gallium from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.73005 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 314 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004337 (456 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_62000
Search term: hitA

Human Homologs

References

Isolation and characterization of gallium resistant Pseudomonas aeruginosa mutants.
García-Contreras R, Lira-Silva E, Jasso-Chávez R, Hernández-González IL, Maeda T, Hashimoto T, Boogerd FC, Sheng L, Wood TK, Moreno-Sánchez R
Int J Med Microbiol 2013 Dec;303(8):574-82
PubMed ID: 23992635
Immunization with the ferric iron-binding periplasmic protein HitA provides protection against Pseudomonas aeruginosa in the murine infection model.
Elhosary MA, Bahey-El-Din M, AbdelBary A, El Guink N, Aboushleib HM
Microb Pathog 2019 Jun;131:181-185
PubMed ID: 30978430