Pseudomonas aeruginosa UCBPP-PA14, PA14_63920 (cntM)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006)
GCF_000014625.1|latest
Locus Tag
PA14_63920
Name
cntM
Synonym: zrmC
Replicon chromosome
Genomic location 5696238 - 5697539 (- strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs
Commentpart of the Zur-regulated metallophore operon known as cntOLMI (PMID:29214991) or zrmABCD(PMID:28898501).

Cross-References

RefSeq YP_793301.1
GI 116052983
Entrez 4383304
INSDC ABJ14219.1
NCBI Locus Tag PA14_63920
UniParc UPI000045D499
UniProtKB Acc Q02FI5
UniProtKB ID Q02FI5_PSEAB
UniRef100 UniRef100_Q02FI5
UniRef50 UniRef50_Q9HUX5
UniRef90 UniRef90_Q9HUX5

Product

Feature Type CDS
Coding Frame 1
Product Name
CntM
Product Name Confidence: Class 3
Synonyms hypothetical protein
Evidence for Translation
Charge (pH 7) 0.90
Kyte-Doolittle Hydrophobicity Value -0.047
Molecular Weight (kDa) 47.7
Isoelectric Point (pI) 7.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6PBP BIOSYNTHETIC PROTEIN 06/14/19 Pseudopaline Dehydrogenase with (S)-Pseudopaline Soaked 1 hour Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.64 X-RAY DIFFRACTION 99.8
6PBM oxidoreductase, biosynthetic protein 06/14/19 Pseudopaline Dehydrogenase with NADP+ bound Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.57 X-RAY DIFFRACTION 99.8
6PBT BIOSYNTHETIC PROTEIN 06/14/19 Pseudopaline Dehydrogenase with (R)-Pseudopaline Soaked 2 hours Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.18 X-RAY DIFFRACTION 99.8
6C4N OXIDOREDUCTASE 01/12/18 Pseudopaline dehydrogenase (PaODH) - NADP+ bound Pseudomonas aeruginosa PAO1 1.95 X-RAY DIFFRACTION 99.8
6PBN BIOSYNTHETIC PROTEIN 06/14/19 Pseudopaline Dehydrogenase with (R)-Pseudopaline Soaked 1 hour Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.65 X-RAY DIFFRACTION 99.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 11 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004466 (299 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_63920
Search term: cntM
Search term: CntM

Human Homologs

References

Growth of Pseudomonas aeruginosa in zinc poor environments is promoted by a nicotianamine-related metallophore.
Mastropasqua MC, D'Orazio M, Cerasi M, Pacello F, Gismondi A, Canini A, Canuti L, Consalvo A, Ciavardelli D, Chirullo B, Pasquali P, Battistoni A
Mol Microbiol 2017 Nov;106(4):543-561
PubMed ID: 28898501
Pseudomonas aeruginosa zinc uptake in chelating environment is primarily mediated by the metallophore pseudopaline.
Lhospice S, Gomez NO, Ouerdane L, Brutesco C, Ghssein G, Hajjar C, Liratni A, Wang S, Richaud P, Bleves S, Ball G, Borezée-Durant E, Lobinski R, Pignol D, Arnoux P, Voulhoux R
Sci Rep 2017 Dec 7;7(1):17132
PubMed ID: 29214991