Pseudomonas aeruginosa UCBPP-PA14, PA14_65000 (azu)

Gene Feature Overview

Strain	Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006) GCF_000014625.1|latest
Locus Tag	PA14_65000
Name	azu
Replicon	chromosome
Genomic location	5793033 - 5793479 (- strand)
Transposon Mutants	2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs	2 transposon mutants in orthologs

Cross-References

RefSeq	YP_793390.1
GI	116053071
Entrez	4380845
INSDC	ABJ14307.1
NCBI Locus Tag	PA14_65000
UniParc	UPI000045D4B9
UniProtKB Acc	Q02F96
UniProtKB ID	Q02F96_PSEAB
UniRef100	UniRef100_Q02F96
UniRef50	UniRef50_P00282
UniRef90	UniRef90_P00282

Product

Feature Type	CDS
Coding Frame	1
Product Name	azurin
Synonyms
Evidence for Translation
Charge (pH 7)	-0.14
Kyte-Doolittle Hydrophobicity Value	-0.084
Molecular Weight (kDa)	16.0
Isoelectric Point (pI)	6.93

Subcellular localization

Individual Mappings	Localization Confidence PMID Periplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
1JZH	ELECTRON TRANSPORT	09/16/01	Pseudomonas aeruginosa Azurin Ru(tpy)(bpy)(His83)	Pseudomonas aeruginosa	1.7	X-RAY DIFFRACTION	99.2
4JKN	ELECTRON TRANSPORT	03/10/13	Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A	Pseudomonas aeruginosa	1.536	X-RAY DIFFRACTION	99.2
1E67	ELECTRON TRANSPORT	08/09/00	Zn-Azurin from Pseudomonas aeruginosa	PSEUDOMONAS AERUGINOSA	2.14	X-RAY DIFFRACTION	99.2
1VLX	ELECTRON TRANSPORT	10/08/96	STRUCTURE OF ELECTRON TRANSFER (COBALT-PROTEIN)	Pseudomonas aeruginosa	1.9	X-RAY DIFFRACTION	99.2
8F5K	ELECTRON TRANSPORT	11/14/22	Azurin from Pseudomonas aeruginosa, Y72F/Y108F/F110A mutant	Pseudomonas aeruginosa	1.25	X-RAY DIFFRACTION	96.6
8F5L	ELECTRON TRANSPORT	11/14/22	Azurin from Pseudomonas aeruginosa, Y72F/Y108F/F110L mutant	Pseudomonas aeruginosa	1.15	X-RAY DIFFRACTION	96.6
5AZU	ELECTRON TRANSPORT(COPPER BINDING)	06/23/93	CRYSTAL STRUCTURE ANALYSIS OF OXIDIZED PSEUDOMONAS AERUGINOSA AZURIN AT PH 5.5 AND PH 9.0. A PH-INDUCED CONFORMATIONAL TRANSITION INVOLVES A PEPTIDE BOND FLIP	Pseudomonas aeruginosa	1.9	X-RAY DIFFRACTION	99.2
1E65	ELECTRON TRANSPORT	08/08/00	Azurin from Pseudomonas aeruginosa, apo form	PSEUDOMONAS AERUGINOSA	1.85	X-RAY DIFFRACTION	99.2
4HZ1	ELECTRON TRANSPORT	11/14/12	Crystal Structure of Pseudomonas aeruginosa azurin with iron(II) at the copper-binding site.	Pseudomonas aeruginosa	2.2	X-RAY DIFFRACTION	99.2
4AZU	ELECTRON TRANSPORT(COPPER BINDING)	06/23/93	CRYSTAL STRUCTURE ANALYSIS OF OXIDIZED PSEUDOMONAS AERUGINOSA AZURIN AT PH 5.5 AND PH 9.0. A PH-INDUCED CONFORMATIONAL TRANSITION INVOLVES A PEPTIDE BOND FLIP	Pseudomonas aeruginosa	1.9	X-RAY DIFFRACTION	99.2

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name	Source Accession	Source DB	Version	Target Accession	Target Description	Percent Identity	Alignment Length	E-Value
lambda-bis(2,2'-bipyridine)imidazole osmium (II)	DB03492	DrugBank	5.1.4	P00282	Azurin	99.3	148	2.8e-78
1-[Pyrrol-1-Yl-2,5-Dione-Methoxymethyl]-Pyrrole-2,5-Dione	DB04085	DrugBank	5.1.4	P00282	Azurin	99.3	148	2.8e-78
Tetra(Imidazole)Diaquacopper (Ii)	DB03840	DrugBank	5.1.4	P00282	Azurin	99.3	148	2.8e-78
4,7-Dimethyl-[1,10]Phenanthroline	DB02586	DrugBank	5.1.4	P00282	Azurin	99.3	148	2.8e-78
S-Hydroxycysteine	DB01915	DrugBank	5.1.4	P00282	Azurin	99.3	148	2.8e-78
Tetra(Imidazole)Diaquacopper (I)	DB04231	DrugBank	5.1.4	P00282	Azurin	99.3	148	2.8e-78
(1,10 Phenanthroline)-(Tri-Carbon Monoxide) Rhenium (I)	DB04100	DrugBank	5.1.4	P00282	Azurin	99.3	148	2.8e-78
1,1'-HEXANE-1,6-DIYLBIS(1H-IMIDAZOLE)	DB06968	DrugBank	5.1.4	P00282	Azurin	99.3	148	2.8e-78
lambda-bis(2,2'-bipyridine)imidazole ruthenium (II)	DB03871	DrugBank	5.1.4	P00282	Azurin	99.3	148	2.8e-78
Tris-Hydroxymethyl-Methyl-Ammonium	DB03570	DrugBank	5.1.4	P00282	Azurin	99.3	148	2.8e-78

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 57 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004539 (509 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA14_65000 Search term: azu Search term: azurin

Human Homologs

References