Pseudomonas aeruginosa UCBPP-PA14, PA14_68200 (rmlA)

Gene Feature Overview

Strain	Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006) GCF_000014625.1|latest
Locus Tag	PA14_68200
Name	rmlA
Replicon	chromosome
Genomic location	6084412 - 6085293 (+ strand)

Cross-References

RefSeq	YP_793634.1
GI	116053310
Entrez	4383477
INSDC	ABJ14546.1
NCBI Locus Tag	PA14_68200
UniParc	UPI0000396FFC
UniProtKB Acc	Q02EK2
UniProtKB ID	Q02EK2_PSEAB
UniRef100	UniRef100_Q02EK2
UniRef50	UniRef50_P37744
UniRef90	UniRef90_Q9HU22

Product

Feature Type	CDS
Coding Frame	1
Product Name	glucose-1-phosphate thymidylyltransferase
Synonyms
Evidence for Translation
Charge (pH 7)	-4.83
Kyte-Doolittle Hydrophobicity Value	-0.195
Molecular Weight (kDa)	32.5
Isoelectric Point (pI)	5.39

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
1FXO	TRANSFERASE	09/26/00	THE STRUCTURAL BASIS OF THE CATALYTIC MECHANISM AND REGULATION OF GLUCOSE-1-PHOSPHATE THYMIDYLYLTRANSFERASE (RMLA). TMP COMPLEX.	Pseudomonas aeruginosa	1.66	X-RAY DIFFRACTION	99.7
4ASJ	TRANSFERASE	05/01/12	Pseudomonas aeruginosa RmlA in complex with allosteric inhibitor	PSEUDOMONAS AERUGINOSA	2.25	X-RAY DIFFRACTION	99.7
4ASY	TRANSFERASE	05/03/12	Pseudomonas aeruginosa RmlA in complex with allosteric inhibitor	PSEUDOMONAS AERUGINOSA	2.3	X-RAY DIFFRACTION	99.7
1G2V	TRANSFERASE	10/21/00	THE STRUCTURAL BASIS OF THE CATALYTIC MECHANISM AND REGULATION OF GLUCOSE-1-PHOSPHATE THYMIDYLYLTRANSFERASE (RMLA). TTP COMPLEX.	Pseudomonas aeruginosa	2.6	X-RAY DIFFRACTION	99.7
1G1L	TRANSFERASE	10/12/00	THE STRUCTURAL BASIS OF THE CATALYTIC MECHANISM AND REGULATION OF GLUCOSE-1-PHOSPHATE THYMIDYLYLTRANSFERASE (RMLA). TDP-GLUCOSE COMPLEX.	Pseudomonas aeruginosa	1.77	X-RAY DIFFRACTION	99.7
1G23	TRANSFERASE	10/16/00	THE STRUCTURAL BASIS OF THE CATALYTIC MECHANISM AND REGULATION OF GLUCOSE-1-PHOSPHATE THYMIDYLYLTRANSFERASE (RMLA). GLUCOSE-1-PHOSPHATE COMPLEX.	Pseudomonas aeruginosa	2.8	X-RAY DIFFRACTION	99.7

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name	Source Accession	Source DB	Version	Target Accession	Target Description	Percent Identity	Alignment Length	E-Value
2'deoxy-Thymidine-5'-Diphospho-Alpha-D-Glucose	DB03751	DrugBank	5.1.4	Q9HU22	Glucose-1-phosphate thymidylyltransferase	99.7	293	9.7e-168
Thymidine	DB04485	DrugBank	5.1.4	Q9HU22	Glucose-1-phosphate thymidylyltransferase	99.7	293	9.7e-168
Thymidine-5'-Triphosphate	DB02452	DrugBank	5.1.4	Q9HU22	Glucose-1-phosphate thymidylyltransferase	99.7	293	9.7e-168
Citric acid	DB04272	DrugBank	5.1.4	Q9HU22	Glucose-1-phosphate thymidylyltransferase	99.7	293	9.7e-168
Thymidine monophosphate	DB01643	DrugBank	5.1.4	Q9HU22	Glucose-1-phosphate thymidylyltransferase	99.7	293	9.7e-168
alpha-D-glucose-1-phosphate	DB02843	DrugBank	5.1.4	Q9HU22	Glucose-1-phosphate thymidylyltransferase	99.7	293	9.7e-168
2'-Deoxy-Thymidine-Beta-L-Rhamnose	DB03723	DrugBank	5.1.4	Q9HU22	Glucose-1-phosphate thymidylyltransferase	99.7	293	9.7e-168

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 636 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004754 (534 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA14_68200 Search term: rmlA Search term: glucose-1-phosphate thymidylyltransferase

Human Homologs

Ensembl 110, assembly GRCh38.p14	ENSP00000483996 eukaryotic translation initiation factor 2B subunit gamma [Source:HGNC Symbol;Acc:HGNC:3259] E-value: 3.2e-06 Percent Identity: 39.3
Ensembl 110, assembly GRCh38.p14	ENSP00000485842 eukaryotic translation initiation factor 2B subunit gamma [Source:HGNC Symbol;Acc:HGNC:3259] E-value: 3.2e-06 Percent Identity: 39.3
Ensembl 110, assembly GRCh38.p14	ENSP00000361257 eukaryotic translation initiation factor 2B subunit gamma [Source:HGNC Symbol;Acc:HGNC:3259] E-value: 3.2e-06 Percent Identity: 39.3
Ensembl 110, assembly GRCh38.p14	ENSP00000353575 eukaryotic translation initiation factor 2B subunit gamma [Source:HGNC Symbol;Acc:HGNC:3259] E-value: 3.2e-06 Percent Identity: 39.3

References