Pseudomonas aeruginosa PAO1, PA3581 (glpF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3581
Name
glpF
Replicon chromosome
Genomic location 4012806 - 4013645 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252271.1
GI 15598777
Affymetrix PA3581_glpF_at
Entrez 880152
GenBank AAG06969.1
INSDC AAG06969.1
NCBI Locus Tag PA3581
protein_id(GenBank) gb|AAG06969.1|AE004779_1|gnl|PseudoCAP|PA3581
TIGR NTL03PA03581
UniParc UPI000012B776
UniProtKB Acc Q51389
UniProtKB ID GLPF_PSEAE
UniRef100 UniRef100_Q51389
UniRef50 UniRef50_P31140
UniRef90 UniRef90_Q51389

Product

Feature Type CDS
Coding Frame 1
Product Name
glycerol uptake facilitator protein
Product Name Confidence: Class 1
Synonyms Glycerol diffusion facilitator
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.42
Kyte-Doolittle Hydrophobicity Value 0.834
Molecular Weight (kDa) 28.9
Isoelectric Point (pI) 6.24

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 344 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002089 (506 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3581
Search term: glpF

Human Homologs

Ensembl 110, assembly GRCh38.p14
aquaporin 9 [Source:HGNC Symbol;Acc:HGNC:643]
E-value: 3.0e-46
Percent Identity: 42.8

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Structure and gene-polypeptide relationships of the region encoding glycerol diffusion facilitator (glpF) and glycerol kinase (glpK) of Pseudomonas aeruginosa.
Schweizer HP, Jump R, Po C
Microbiology (Reading) 1997 Apr;143 ( Pt 4):1287-1297
PubMed ID: 9141691