Pseudomonas aeruginosa PAO1, PA3637 (pyrG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3637
Name
pyrG
Replicon chromosome
Genomic location 4070860 - 4072488 (- strand)

Cross-References

RefSeq NP_252327.1
GI 15598833
Affymetrix PA3637_pyrG_at
Entrez 880546
GenBank AAG07025.1
INSDC AAG07025.1
NCBI Locus Tag PA3637
protein_id(GenBank) gb|AAG07025.1|AE004783_10|gnl|PseudoCAP|PA3637
TIGR NTL03PA03637
UniParc UPI0000132D75
UniProtKB Acc Q9HXZ4
UniProtKB ID PYRG_PSEAE
UniRef100 UniRef100_A6V1F1
UniRef50 UniRef50_P28595
UniRef90 UniRef90_A6V1F1

Product

Feature Type CDS
Coding Frame 1
Product Name
CTP synthase
Product Name Confidence: Class 2
Synonyms UTP--ammonia ligase
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by iTRAQ (PMID:21863142).
Charge (pH 7) -13.54
Kyte-Doolittle Hydrophobicity Value -0.152
Molecular Weight (kDa) 59.6
Isoelectric Point (pI) 5.38

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 655 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002037 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3637
Search term: pyrG
Search term: CTP synthase

Human Homologs

Ensembl 110, assembly GRCh38.p14
CTP synthase 1 [Source:HGNC Symbol;Acc:HGNC:2519]
E-value: 1.7e-138
Percent Identity: 44.8
Ensembl 110, assembly GRCh38.p14
CTP synthase 1 [Source:HGNC Symbol;Acc:HGNC:2519]
E-value: 1.7e-138
Percent Identity: 44.8
Ensembl 110, assembly GRCh38.p14
CTP synthase 1 [Source:HGNC Symbol;Acc:HGNC:2519]
E-value: 1.7e-138
Percent Identity: 44.8
Ensembl 110, assembly GRCh38.p14
CTP synthase 1 [Source:HGNC Symbol;Acc:HGNC:2519]
E-value: 1.7e-138
Percent Identity: 44.8
Ensembl 110, assembly GRCh38.p14
CTP synthase 1 [Source:HGNC Symbol;Acc:HGNC:2519]
E-value: 1.7e-138
Percent Identity: 44.8

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Nucleotide sequence of Escherichia coli pyrG encoding CTP synthetase.
Weng M, Makaroff CA, Zalkin H
J Biol Chem 1986 Apr 25;261(12):5568-74
PubMed ID: 3514618
Structural role for a conserved region in the CTP synthetase glutamine amide transfer domain.
Weng ML, Zalkin H
J Bacteriol 1987 Jul;169(7):3023-8
PubMed ID: 3298209
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142