Pseudomonas aeruginosa PAO1, PA3640 (dnaE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3640
Name
dnaE
Synonym: polC
Replicon chromosome
Genomic location 4075157 - 4078678 (- strand)

Cross-References

RefSeq NP_252330.1
GI 15598836
Affymetrix PA3640_dnaE_at
Entrez 880490
GenBank AAG07028.1
INSDC AAG16710.2
NCBI Locus Tag PA3640
protein_id(GenBank) gb|AAG07028.1|AE004784_1|gnl|PseudoCAP|PA3640
TIGR NTL03PA03640
UniParc UPI00001296DE
UniProtKB Acc Q9HXZ1
UniProtKB ID DPO3A_PSEAE
UniRef100 UniRef100_Q9HXZ1
UniRef50 UniRef50_P10443
UniRef90 UniRef90_Q9HXZ1

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA polymerase III, alpha chain
Product Name Confidence: Class 2
Synonyms DNA nucleotidyltransferase (DNA-directed)
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -30.23
Kyte-Doolittle Hydrophobicity Value -0.237
Molecular Weight (kDa) 130.9
Isoelectric Point (pI) 5.07

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 591 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002034 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3640
Search term: dnaE

Human Homologs

References

The polymerase subunit of DNA polymerase III of Escherichia coli. I. Amplification of the dnaE gene product and polymerase activity of the alpha subunit.
Maki H, Horiuchi T, Kornberg A
J Biol Chem 1985 Oct 25;260(24):12982-6
PubMed ID: 2932432
Sequence analysis of the Escherichia coli dnaE gene.
Tomasiewicz HG, McHenry CS
J Bacteriol 1987 Dec;169(12):5735-44
PubMed ID: 3316192
Antimutator mutations in the alpha subunit of Escherichia coli DNA polymerase III: identification of the responsible mutations and alignment with other DNA polymerases.
Fijalkowska IJ, Schaaper RM
Genetics 1993 Aug;134(4):1039-44
PubMed ID: 8375647