Pseudomonas aeruginosa PAO1, PA3642 (rnhB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3642
Name
rnhB
Replicon chromosome
Genomic location 4080440 - 4081045 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252332.1
GI 15598838
Affymetrix PA3642_rnhB_at
DNASU PaCD00007597
Entrez 880498
GenBank AAG07030.1
INSDC AAG07030.1
NCBI Locus Tag PA3642
protein_id(GenBank) gb|AAG07030.1|AE004784_3|gnl|PseudoCAP|PA3642
TIGR NTL03PA03642
UniParc UPI00000C5AA2
UniProtKB Acc Q9HXY9
UniProtKB ID RNH2_PSEAE
UniRef100 UniRef100_Q9HXY9
UniRef50 UniRef50_Q9HXY9
UniRef90 UniRef90_Q9HXY9

Product

Feature Type CDS
Coding Frame 1
Product Name
ribonuclease HII
Product Name Confidence: Class 2
Synonyms RNAse HII
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.16
Kyte-Doolittle Hydrophobicity Value 0.122
Molecular Weight (kDa) 21.8
Isoelectric Point (pI) 6.52

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 634 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002032 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3642
Search term: rnhB
Search term: ribonuclease HII

Human Homologs

References

Isolation and characterization of a second RNase H (RNase HII) of Escherichia coli K-12 encoded by the rnhB gene.
Itaya M
Proc Natl Acad Sci U S A 1990 Nov;87(21):8587-91
PubMed ID: 2172991