Pseudomonas aeruginosa PAO1, PA3646 (lpxD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3646
Name
lpxD
Synonym: firA
Synonym: omsA
Replicon chromosome
Genomic location 4083444 - 4084505 (- strand)

Cross-References

RefSeq NP_252336.1
GI 15598842
Affymetrix PA3646_lpxD_at
Entrez 880525
GenBank AAG07034.1
INSDC AAG07034.1
NCBI Locus Tag PA3646
protein_id(GenBank) gb|AAG07034.1|AE004784_7|gnl|PseudoCAP|PA3646
TIGR NTL03PA03646
UniParc UPI000012E8FE
UniProtKB Acc Q9HXY6
UniProtKB ID LPXD_PSEAE
UniRef100 UniRef100_B7V7U2
UniRef50 UniRef50_B0VMV2
UniRef90 UniRef90_B7V7U2

Product

Feature Type CDS
Coding Frame 1
Product Name
UDP-3-O-[3-hydroxylauroyl] glucosamine N-acyltransferase
Product Name Confidence: Class 2
Synonyms FirA protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.96
Kyte-Doolittle Hydrophobicity Value 0.187
Molecular Weight (kDa) 36.2
Isoelectric Point (pI) 6.23

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6UED TRANSFERASE 09/20/19 Apo Pseudomonas aeruginosa LpxD Structure Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.55 X-RAY DIFFRACTION 100.0
6UEC TRANSFERASE 09/20/19 Pseudomonas aeruginosa LpxD Complex Structure with Ligand Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.6 X-RAY DIFFRACTION 100.0
3PMO TRANSFERASE 11/17/10 The structure of LpxD from Pseudomonas aeruginosa at 1.3 A resolution Pseudomonas aeruginosa 1.3 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 412 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002028 (520 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3646
Search term: lpxD

Human Homologs

References

Structural characterization of the lipid A component of Pseudomonas aeruginosa wild-type and rough mutant lipopolysaccharides.
Kulshin VA, Zähringer U, Lindner B, Jäger KE, Dmitriev BA, Rietschel ET
Eur J Biochem 1991 Jun 15;198(3):697-704
PubMed ID: 1904818
Defective biosynthesis of the lipid A component of temperature-sensitive firA (omsA) mutant of Escherichia coli.
Helander IM, Lindner B, Seydel U, Vaara M
Eur J Biochem 1993 Mar 1;212(2):363-9
PubMed ID: 8444173
The firA gene of Escherichia coli encodes UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase. The third step of endotoxin biosynthesis.
Kelly TM, Stachula SA, Raetz CR, Anderson MS
J Biol Chem 1993 Sep 15;268(26):19866-74
PubMed ID: 8366125
The novel hexapeptide motif found in the acyltransferases LpxA and LpxD of lipid A biosynthesis is conserved in various bacteria.
Vuorio R, Härkönen T, Tolvanen M, Vaara M
FEBS Lett 1994 Jan 17;337(3):289-92
PubMed ID: 8293817