Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3646
|
Name |
lpxD
Synonym: firA Synonym: omsA |
Replicon | chromosome |
Genomic location | 4083444 - 4084505 (- strand) |
RefSeq | NP_252336.1 |
GI | 15598842 |
Affymetrix | PA3646_lpxD_at |
Entrez | 880525 |
GenBank | AAG07034.1 |
INSDC | AAG07034.1 |
NCBI Locus Tag | PA3646 |
protein_id(GenBank) | gb|AAG07034.1|AE004784_7|gnl|PseudoCAP|PA3646 |
TIGR | NTL03PA03646 |
UniParc | UPI000012E8FE |
UniProtKB Acc | Q9HXY6 |
UniProtKB ID | LPXD_PSEAE |
UniRef100 | UniRef100_B7V7U2 |
UniRef50 | UniRef50_B0VMV2 |
UniRef90 | UniRef90_B7V7U2 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
UDP-3-O-[3-hydroxylauroyl] glucosamine N-acyltransferase
|
Synonyms |
FirA protein |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -3.96 |
Kyte-Doolittle Hydrophobicity Value | 0.187 |
Molecular Weight (kDa) | 36.2 |
Isoelectric Point (pI) | 6.23 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6UED | TRANSFERASE | 09/20/19 | Apo Pseudomonas aeruginosa LpxD Structure | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.55 | X-RAY DIFFRACTION | 100.0 |
6UEC | TRANSFERASE | 09/20/19 | Pseudomonas aeruginosa LpxD Complex Structure with Ligand | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 2.6 | X-RAY DIFFRACTION | 100.0 |
3PMO | TRANSFERASE | 11/17/10 | The structure of LpxD from Pseudomonas aeruginosa at 1.3 A resolution | Pseudomonas aeruginosa | 1.3 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 412 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG002028 (520 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3646
Search term: lpxD
|
Structural characterization of the lipid A component of Pseudomonas aeruginosa wild-type and rough mutant lipopolysaccharides.
Kulshin VA, Zähringer U, Lindner B, Jäger KE, Dmitriev BA, Rietschel ET
Eur J Biochem 1991 Jun 15;198(3):697-704
PubMed ID: 1904818
|
Defective biosynthesis of the lipid A component of temperature-sensitive firA (omsA) mutant of Escherichia coli.
Helander IM, Lindner B, Seydel U, Vaara M
Eur J Biochem 1993 Mar 1;212(2):363-9
PubMed ID: 8444173
|
The firA gene of Escherichia coli encodes UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase. The third step of endotoxin biosynthesis.
Kelly TM, Stachula SA, Raetz CR, Anderson MS
J Biol Chem 1993 Sep 15;268(26):19866-74
PubMed ID: 8366125
|
The novel hexapeptide motif found in the acyltransferases LpxA and LpxD of lipid A biosynthesis is conserved in various bacteria.
Vuorio R, Härkönen T, Tolvanen M, Vaara M
FEBS Lett 1994 Jan 17;337(3):289-92
PubMed ID: 8293817
|