Pseudomonas aeruginosa PAO1, PA3653 (frr)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3653
Name
frr
Synonym: rrf
Replicon chromosome
Genomic location 4091671 - 4092228 (- strand)

Cross-References

RefSeq NP_252343.1
GI 15598849
Affymetrix PA3653_frr_at
Entrez 880554
GenBank AAG07041.1
INSDC AAG07041.1
NCBI Locus Tag PA3653
protein_id(GenBank) gb|AAG07041.1|AE004785_5|gnl|PseudoCAP|PA3653
TIGR NTL03PA03653
UniParc UPI0000134A39
UniProtKB Acc O82853
UniProtKB ID RRF_PSEAE
UniRef100 UniRef100_B7V7G0
UniRef50 UniRef50_P81101
UniRef90 UniRef90_A6V1D5

Product

Feature Type CDS
Coding Frame 1
Product Name
ribosome recycling factor
Product Name Confidence: Class 1
Synonyms ribosome releasing factor
Evidence for Translation
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.51
Kyte-Doolittle Hydrophobicity Value -0.464
Molecular Weight (kDa) 20.5
Isoelectric Point (pI) 5.91

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 593 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002021 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3653
Search term: frr

Human Homologs

References

Molecular cloning and expression of ribosome releasing factor.
Ichikawa S, Kaji A
J Biol Chem 1989 Nov 25;264(33):20054-9
PubMed ID: 2684966
Ribosome recycling factor (ribosome releasing factor) is essential for bacterial growth.
Janosi L, Shimizu I, Kaji A
Proc Natl Acad Sci U S A 1994 May 10;91(10):4249-53
PubMed ID: 8183897
Molecular cloning, sequencing, purification, and characterization of Pseudomonas aeruginosa ribosome recycling factor.
Ohnishi M, Janosi L, Shuda M, Matsumoto H, Hayashi T, Terawaki Y, Kaji A
J Bacteriol 1999 Feb;181(4):1281-91
PubMed ID: 9973356
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142