Pseudomonas aeruginosa PAO1, PA3655 (tsf)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3655
Name
tsf
Replicon chromosome
Genomic location 4093167 - 4094036 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_252345.1
GI 15598851
Affymetrix PA3655_tsf_at
DNASU PaCD00007602
Entrez 880592
GenBank AAG07043.1
INSDC AAG07043.1
NCBI Locus Tag PA3655
protein_id(GenBank) gb|AAG07043.1|AE004785_7|gnl|PseudoCAP|PA3655
TIGR NTL03PA03655
UniParc UPI0000129D5B
UniProtKB Acc O82851
UniProtKB ID EFTS_PSEAE
UniRef100 UniRef100_A6V1D3
UniRef50 UniRef50_Q4QLZ5
UniRef90 UniRef90_A6V1D3

Product

Feature Type CDS
Coding Frame 1
Product Name
elongation factor Ts
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -7.29
Kyte-Doolittle Hydrophobicity Value -0.020
Molecular Weight (kDa) 30.7
Isoelectric Point (pI) 4.95

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 591 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002019 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3655
Search term: tsf

Human Homologs

References

Organization and nucleotide sequence of a new ribosomal operon in Escherichia coli containing the genes for ribosomal protein S2 and elongation factor Ts.
An G, Bendiak DS, Mamelak LA, Friesen JD
Nucleic Acids Res 1981 Aug 25;9(16):4163-72
PubMed ID: 6272196
The structure of the Escherichia coli EF-Tu.EF-Ts complex at 2.5 A resolution.
Kawashima T, Berthet-Colominas C, Wulff M, Cusack S, Leberman R
Nature 1996 Feb 8;379(6565):511-8
PubMed ID: 8596629
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994