Pseudomonas aeruginosa PAO1, PA3656 (rpsB)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3656
Name	rpsB
Replicon	chromosome
Genomic location	4094167 - 4094907 (- strand)

Cross-References

RefSeq	NP_252346.1
GI	15598852
Affymetrix	PA3656_rpsB_at
DNASU	PaCD00007603
Entrez	880593
GenBank	AAG07044.1
INSDC	AAG07044.1
NCBI Locus Tag	PA3656
protein_id(GenBank)	gb|AAG07044.1|AE004785_8|gnl|PseudoCAP|PA3656
TIGR	NTL03PA03656
UniParc	UPI0000134F14
UniProtKB Acc	O82850
UniProtKB ID	RS2_PSEAE
UniRef100	UniRef100_B7V7F7
UniRef50	UniRef50_Q0AYK4
UniRef90	UniRef90_B7V7F7

Product

Feature Type	CDS
Coding Frame	1
Product Name	30S ribosomal protein S2 Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Detected by MALDI-TOF/TOF (PMID:19333994). Detected using LC-MS/MS (27.34 kDa, PMID:21751344) Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	3.17
Kyte-Doolittle Hydrophobicity Value	-0.335
Molecular Weight (kDa)	27.3
Isoelectric Point (pI)	8.95

Subcellular localization

Individual Mappings	Localization Confidence PMID Outer Membrane Vesicle Class 1 Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
7UNU	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
6SPG	RIBOSOME	09/01/19	Pseudomonas aeruginosa 70s ribosome from a clinical isolate	Pseudomonas aeruginosa	3.34	ELECTRON MICROSCOPY	100.0
7UNV	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.7	ELECTRON MICROSCOPY	100.0
7UNR	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
6SPF	RIBOSOME	09/01/19	Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate	Pseudomonas aeruginosa	2.89	ELECTRON MICROSCOPY	100.0
6SPE	RIBOSOME	09/01/19	Pseudomonas aeruginosa 30s ribosome from a clinical isolate	Pseudomonas aeruginosa	3.6	ELECTRON MICROSCOPY	100.0
7UNW	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.6	ELECTRON MICROSCOPY	100.0
6SPC	RIBOSOME	09/01/19	Pseudomonas aeruginosa 30s ribosome from an aminoglycoside resistant clinical isolate	Pseudomonas aeruginosa	2.95	ELECTRON MICROSCOPY	97.8

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 607 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002018 (537 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3656 Search term: rpsB Search term: 30S ribosomal protein S2

Human Homologs

References

Physical studies on the ribosomal protein S2 from the Escherichia coli 30S. Georgalis Y, Giri L, Littlechild JA Biochemistry 1981 Mar 3;20(5):1061-4 PubMed ID: 7013783

Probing the assembly of the 3' major domain of 16 S rRNA. Interactions involving ribosomal proteins S2, S3, S10, S13 and S14. Powers T, Stern S, Changchien LM, Noller HF J Mol Biol 1988 Jun 20;201(4):697-716 PubMed ID: 2459390

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa. Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS Proteomics 2011 Aug;11(16):3424-9 PubMed ID: 21751344