Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3657
|
Name |
map
|
Replicon | chromosome |
Genomic location | 4095172 - 4095957 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_252347.1 |
GI | 15598853 |
Affymetrix | PA3657_map_at |
Entrez | 880564 |
GenBank | AAG07045.1 |
INSDC | AAG07045.1 |
NCBI Locus Tag | PA3657 |
protein_id(GenBank) | gb|AAG07045.1|AE004785_9|gnl|PseudoCAP|PA3657 |
TIGR | NTL03PA03657 |
UniParc | UPI00000C5AA5 |
UniProtKB Acc | Q9HXY1 |
UniProtKB ID | Q9HXY1_PSEAE |
UniRef100 | UniRef100_W0YTW9 |
UniRef50 | UniRef50_A6V1D1 |
UniRef90 | UniRef90_A6V1D1 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
methionine aminopeptidase
|
Synonyms |
peptidase M MAP |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
|
Charge (pH 7) | -4.53 |
Kyte-Doolittle Hydrophobicity Value | -0.308 |
Molecular Weight (kDa) | 29.1 |
Isoelectric Point (pI) | 6.29 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
4JUQ | HYDROLASE | 03/25/13 | Pseudomonas aeruginosa MetAP T2N mutant, in Mn form | Pseudomonas aeruginosa | 2.2 | X-RAY DIFFRACTION | 99.6 |
4FO8 | HYDROLASE | 06/20/12 | Pseudomonas aeruginosa MetAP with Met, in Mn form | Pseudomonas aeruginosa | 1.9 | X-RAY DIFFRACTION | 100.0 |
4FO7 | HYDROLASE | 06/20/12 | Pseudomonas aeruginosa MetAP, in Mn form | Pseudomonas aeruginosa | 1.8 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 665 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG002017 (538 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3657
Search term: map
Search term: methionine aminopeptidase
|
Ensembl
110, assembly
GRCh38.p14
|
methionyl aminopeptidase 1 [Source:HGNC Symbol;Acc:HGNC:15789]
E-value:
2.9e-75
Percent Identity:
52.0
|
Purification and characterization of a methionine-specific aminopeptidase from Salmonella typhimurium.
Wingfield P, Graber P, Turcatti G, Movva NR, Pelletier M, Craig S, Rose K, Miller CG
Eur J Biochem 1989 Mar 1;180(1):23-32
PubMed ID: 2651123
|
pepM is an essential gene in Salmonella typhimurium.
Miller CG, Kukral AM, Miller JL, Movva NR
J Bacteriol 1989 Sep;171(9):5215-7
PubMed ID: 2670909
|
Structure of the cobalt-dependent methionine aminopeptidase from Escherichia coli: a new type of proteolytic enzyme.
Roderick SL, Matthews BW
Biochemistry 1993 Apr 20;32(15):3907-12
PubMed ID: 8471602
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|