Pseudomonas aeruginosa PAO1, PA3657 (map)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3657
Name
map
Replicon chromosome
Genomic location 4095172 - 4095957 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252347.1
GI 15598853
Affymetrix PA3657_map_at
Entrez 880564
GenBank AAG07045.1
INSDC AAG07045.1
NCBI Locus Tag PA3657
protein_id(GenBank) gb|AAG07045.1|AE004785_9|gnl|PseudoCAP|PA3657
TIGR NTL03PA03657
UniParc UPI00000C5AA5
UniProtKB Acc Q9HXY1
UniProtKB ID Q9HXY1_PSEAE
UniRef100 UniRef100_W0YTW9
UniRef50 UniRef50_A6V1D1
UniRef90 UniRef90_A6V1D1

Product

Feature Type CDS
Coding Frame 1
Product Name
methionine aminopeptidase
Product Name Confidence: Class 2
Synonyms peptidase M
MAP
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Charge (pH 7) -4.53
Kyte-Doolittle Hydrophobicity Value -0.308
Molecular Weight (kDa) 29.1
Isoelectric Point (pI) 6.29

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4JUQ HYDROLASE 03/25/13 Pseudomonas aeruginosa MetAP T2N mutant, in Mn form Pseudomonas aeruginosa 2.2 X-RAY DIFFRACTION 99.6
4FO8 HYDROLASE 06/20/12 Pseudomonas aeruginosa MetAP with Met, in Mn form Pseudomonas aeruginosa 1.9 X-RAY DIFFRACTION 100.0
4FO7 HYDROLASE 06/20/12 Pseudomonas aeruginosa MetAP, in Mn form Pseudomonas aeruginosa 1.8 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 665 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002017 (538 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3657
Search term: map

Human Homologs

Ensembl 110, assembly GRCh38.p14
methionyl aminopeptidase 1 [Source:HGNC Symbol;Acc:HGNC:15789]
E-value: 2.9e-75
Percent Identity: 52.0

References

Purification and characterization of a methionine-specific aminopeptidase from Salmonella typhimurium.
Wingfield P, Graber P, Turcatti G, Movva NR, Pelletier M, Craig S, Rose K, Miller CG
Eur J Biochem 1989 Mar 1;180(1):23-32
PubMed ID: 2651123
pepM is an essential gene in Salmonella typhimurium.
Miller CG, Kukral AM, Miller JL, Movva NR
J Bacteriol 1989 Sep;171(9):5215-7
PubMed ID: 2670909
Structure of the cobalt-dependent methionine aminopeptidase from Escherichia coli: a new type of proteolytic enzyme.
Roderick SL, Matthews BW
Biochemistry 1993 Apr 20;32(15):3907-12
PubMed ID: 8471602
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142