Pseudomonas aeruginosa PAO1, PA3700 (lysS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3700
Name
lysS
Replicon chromosome
Genomic location 4140884 - 4142389 (- strand)

Cross-References

RefSeq NP_252390.1
GI 15598896
Affymetrix PA3700_lysS_at
Entrez 880383
GenBank AAG07088.1
INSDC AAG07088.1
NCBI Locus Tag PA3700
protein_id(GenBank) gb|AAG07088.1|AE004789_8|gnl|PseudoCAP|PA3700
TIGR NTL03PA03700
UniParc UPI00000C5AC8
UniProtKB Acc Q9HXU0
UniProtKB ID SYK_PSEAE
UniRef100 UniRef100_Q9HXU0
UniRef50 UniRef50_Q9HXU0
UniRef90 UniRef90_Q9HXU0

Product

Feature Type CDS
Coding Frame 1
Product Name
lysyl-tRNA synthetase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -17.59
Kyte-Doolittle Hydrophobicity Value -0.444
Molecular Weight (kDa) 57.3
Isoelectric Point (pI) 5.04

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 668 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001977 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3700
Search term: lysS

Human Homologs

Ensembl 110, assembly GRCh38.p14
lysyl-tRNA synthetase 1 [Source:HGNC Symbol;Acc:HGNC:6215]
E-value: 4.5e-93
Percent Identity: 40.2
Ensembl 110, assembly GRCh38.p14
lysyl-tRNA synthetase 1 [Source:HGNC Symbol;Acc:HGNC:6215]
E-value: 4.5e-93
Percent Identity: 40.2

References

Homology of lysS and lysU, the two Escherichia coli genes encoding distinct lysyl-tRNA synthetase species.
Lévêque F, Plateau P, Dessen P, Blanquet S
Nucleic Acids Res 1990 Jan 25;18(2):305-12
PubMed ID: 2183178
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994