Pseudomonas aeruginosa PAO1, PA3702 (wspR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3702
Name
wspR
Replicon chromosome
Genomic location 4143788 - 4144831 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 6 transposon mutants in orthologs
CommentOverexpression of PA3702 causes increased pellicle formation.

Cross-References

RefSeq NP_252391.1
GI 15598897
Affymetrix PA3702_at
Entrez 878337
GenBank AAG07089.1
INSDC AAG07089.1
NCBI Locus Tag PA3702
protein_id(GenBank) gb|AAG07089.1|AE004789_9|gnl|PseudoCAP|PA3702
TIGR NTL03PA03702
UniParc UPI00000C5AC9
UniProtKB Acc Q9HXT9
UniProtKB ID Q9HXT9_PSEAE
UniRef100 UniRef100_A0A0A8REW0
UniRef50 UniRef50_Q886S7
UniRef90 UniRef90_A0A077JNP3

Product

Feature Type CDS
Coding Frame 1
Product Name
WspR
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.13
Kyte-Doolittle Hydrophobicity Value -0.229
Molecular Weight (kDa) 37.9
Isoelectric Point (pI) 5.64

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3I5B SIGNALING PROTEIN 07/03/09 Crystal structure of the isolated GGDEF domain of WpsR from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.043 X-RAY DIFFRACTION 100.0
3BRE SIGNALING PROTEIN 12/21/07 Crystal Structure of P.aeruginosa PA3702 Pseudomonas aeruginosa 2.4 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 600 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000167 (2758 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3702
Search term: wspR
Search term: WspR

Human Homologs

References

Autolysis and autoaggregation in Pseudomonas aeruginosa colony morphology mutants.
D'Argenio DA, Calfee MW, Rainey PB, Pesci EC
J Bacteriol 2002 Dec;184(23):6481-9
PubMed ID: 12426335
Phosphorylation-independent regulation of the diguanylate cyclase WspR.
De N, Pirruccello M, Krasteva PV, Bae N, Raghavan RV, Sondermann H
PLoS Biol 2008 Mar 25;6(3):e67
PubMed ID: 18366254
A chemosensory system that regulates biofilm formation through modulation of cyclic diguanylate levels.
Hickman JW, Tifrea DF, Harwood CS
Proc Natl Acad Sci U S A 2005 Oct 4;102(40):14422-7
PubMed ID: 16186483
Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence.
Kulasakara H, Lee V, Brencic A, Liberati N, Urbach J, Miyata S, Lee DG, Neely AN, Hyodo M, Hayakawa Y, Ausubel FM, Lory S
Proc Natl Acad Sci U S A 2006 Feb 21;103(8):2839-44
PubMed ID: 16477007