Pseudomonas aeruginosa PAO1, PA3742 (rplS)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3742
Name	rplS
Replicon	chromosome
Genomic location	4195008 - 4195358 (- strand)
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_252431.1
GI	15598937
Affymetrix	PA3742_rplS_at
DNASU	PaCD00007633
Entrez	880339
GenBank	AAG07129.1
INSDC	AAG07129.1
NCBI Locus Tag	PA3742
protein_id(GenBank)	gb|AAG07129.1|AE004793_6|gnl|PseudoCAP|PA3742
TIGR	NTL03PA03742
UniParc	UPI0000133CB9
UniProtKB Acc	Q9HXQ2
UniProtKB ID	RL19_PSEAE
UniRef100	UniRef100_A6V126
UniRef50	UniRef50_Q87LT1
UniRef90	UniRef90_A4XXT4

Product

Feature Type	CDS
Coding Frame	1
Product Name	50S ribosomal protein L19 Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	11.98
Kyte-Doolittle Hydrophobicity Value	-0.365
Molecular Weight (kDa)	13.0
Isoelectric Point (pI)	11.08

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
7UNR	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
7UNV	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.7	ELECTRON MICROSCOPY	100.0
6SPG	RIBOSOME	09/01/19	Pseudomonas aeruginosa 70s ribosome from a clinical isolate	Pseudomonas aeruginosa	3.34	ELECTRON MICROSCOPY	100.0
7UNW	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.6	ELECTRON MICROSCOPY	100.0
6SPF	RIBOSOME	09/01/19	Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate	Pseudomonas aeruginosa	2.89	ELECTRON MICROSCOPY	100.0
6SPD	RIBOSOME	09/01/19	Pseudomonas aeruginosa 50s ribosome from a clinical isolate	Pseudomonas aeruginosa	3.28	ELECTRON MICROSCOPY	100.0
6SPB	RIBOSOME	09/01/19	Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6	Pseudomonas aeruginosa	2.82	ELECTRON MICROSCOPY	100.0
7UNU	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 592 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001940 (537 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3742 Search term: rplS Search term: 50S ribosomal protein L19

Human Homologs

References

Primary structure of protein L19 from the large subunit of Escherichia coli ribosomes. Brosius J, Arfsten U Biochemistry 1978 Feb 7;17(3):508-16 PubMed ID: 339951

The nucleotide sequence of an Escherichia coli operon containing genes for the tRNA(m1G)methyltransferase, the ribosomal proteins S16 and L19 and a 21-K polypeptide. Byström AS, Hjalmarsson KJ, Wikström PM, Björk GR EMBO J 1983;2(6):899-905 PubMed ID: 6357787

The localization of protein L19 on the surface of 50 S subunits of Escherichia coli aided by the use of mutants lacking protein L19. Stöffler G, Noah M, Stöffler-Meilicke M, Dabbs ER J Biol Chem 1984 Apr 10;259(7):4521-6 PubMed ID: 6423642