Pseudomonas aeruginosa PAO1, PA3805 (pilF)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
| Locus Tag |
PA3805
|
| Name |
pilF
|
| Replicon | chromosome |
| Genomic location | 4264530 - 4265288 (- strand) |
| Transposon Mutants | 1 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Cross-References
| RefSeq | NP_252494.1 |
| GI | 15599000 |
| Affymetrix | PA3805_pilF_at |
| Entrez | 879170 |
| GenBank | AAG07192.1 |
| INSDC | AAG07192.1 |
| NCBI Locus Tag | PA3805 |
| protein_id(GenBank) | gb|AAG07192.1|AE004798_15|gnl|PseudoCAP|PA3805 |
| TIGR | NTL03PA03805 |
| UniParc | UPI00000C5B1F |
| UniProtKB Acc | Q9HXJ2 |
| UniProtKB ID | Q9HXJ2_PSEAE |
| UniRef100 | UniRef100_Q9HXJ2 |
| UniRef50 | UniRef50_Q9HXJ2 |
| UniRef90 | UniRef90_Q9HXJ2 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
type 4 fimbrial biogenesis protein PilF
|
| Synonyms |
pilin biosynthetic gene PilF |
| Evidence for Translation |
Detected using LC-MS/MS (28.54 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
| Charge (pH 7) | 0.21 |
| Kyte-Doolittle Hydrophobicity Value | -0.544 |
| Molecular Weight (kDa) | 28.5 |
| Isoelectric Point (pI) | 7.33 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 2HO1 | PROTEIN BINDING | 07/13/06 | Functional Characterization of Pseudomonas Aeruginosa pilF | Pseudomonas aeruginosa | 2 | X-RAY DIFFRACTION | 100.0 |
| 2FI7 | PROTEIN BINDING | 12/28/05 | Crystal Structure of PilF : Functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa | Pseudomonas aeruginosa | 2.2 | X-RAY DIFFRACTION | 100.0 |
Virulence Evidence
| Source 1 | |
| Database | VFDB : VF0082 |
| Category | Type IV pili biosynthesis |
| Evidence |
|
| Host Organism | |
| Notes | |
| Reference | |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 204 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG001861 (534 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA3805
Search term: pilF
Search term: type 4 fimbrial biogenesis protein PilF
|
Human Homologs
References
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
|
Identification of a gene, pilF, required for type 4 fimbrial biogenesis and twitching motility in Pseudomonas aeruginosa.
Watson AA, Alm RA, Mattick JS
Gene 1996 Nov 21;180(1-2):49-56
PubMed ID: 8973346
|
|
PilF is an outer membrane lipoprotein required for multimerization and localization of the Pseudomonas aeruginosa Type IV pilus secretin.
Koo J, Tammam S, Ku SY, Sampaleanu LM, Burrows LL, Howell PL
J Bacteriol 2008 Nov;190(21):6961-9
PubMed ID: 18776008
|
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|