Pseudomonas aeruginosa PAO1, PA3822

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3822
Name
Synonym: yajC
Replicon chromosome
Genomic location 4279007 - 4279345 (- strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252511.1
GI 15599017
Affymetrix PA3822_at
DNASU PaCD00007665
Entrez 878280
GenBank AAG07209.1
INSDC AAG07209.1
NCBI Locus Tag PA3822
protein_id(GenBank) gb|AAG07209.1|AE004799_15|gnl|PseudoCAP|PA3822
TIGR NTL03PA03822
UniParc UPI00000C5B29
UniProtKB Acc Q9HXI0
UniProtKB ID Q9HXI0_PSEAE
UniRef100 UniRef100_W0YVN4
UniRef50 UniRef50_Q4K6T0
UniRef90 UniRef90_M4X3P6

Product

Feature Type CDS
Coding Frame 1
Product Name
conserved hypothetical protein
Product Name Confidence: Class 4
Synonyms
Evidence for Translation
Detected using LC-MS/MS (11.86 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.22
Kyte-Doolittle Hydrophobicity Value 0.388
Molecular Weight (kDa) 11.9
Isoelectric Point (pI) 9.61

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
YajC YajC interacts with the AcrAB-TolC efflux pump in a way that in uncharacterized but is shown to grant increased fitness in the presence of linezolid, rifampicin, and vancomycin. [PMID:31871033] ARO:3005040 protein homolog model Perfect 217.2 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 453 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001844 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3822

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Genetic and molecular characterization of the Escherichia coli secD operon and its products.
Pogliano KJ, Beckwith J
J Bacteriol 1994 Feb;176(3):804-14
PubMed ID: 7507921
Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme.
Duong F, Wickner W
EMBO J 1997 May 15;16(10):2756-68
PubMed ID: 9184221
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344