Pseudomonas aeruginosa PAO1, PA3865

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3865
Name
Replicon	chromosome
Genomic location	4325604 - 4326395 (- strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs
Comment	Structural similarity to 1LAF (Lys/Arg/His-binding protein from Salmonella typhimurium, root-mean-square deviation:0.4 angstroms). Sequence similarity to 6FT2 (98%/46,58%) (ARG). Final predicted ligand: basic amino acid (PMID: 31631799)

Cross-References

RefSeq	NP_252554.1
GI	15599060
Affymetrix	PA3865_at
DNASU	PaCD00007683
Entrez	879821
GenBank	AAG07252.1
INSDC	AAG07252.1
NCBI Locus Tag	PA3865
protein_id(GenBank)	gb|AAG07252.1|AE004803_8|gnl|PseudoCAP|PA3865
TIGR	NTL03PA03865
UniParc	UPI00000C5B4D
UniProtKB Acc	Q9HXE1
UniProtKB ID	Q9HXE1_PSEAE
UniRef100	UniRef100_Q9HXE1
UniRef50	UniRef50_Q3KEJ9
UniRef90	UniRef90_V6AAF0

Product

Feature Type	CDS
Coding Frame	1
Product Name	putative periplasmic lysine-, arginine-, ornithine-binding protein Product Name Confidence: Class 3
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7)	-0.82
Kyte-Doolittle Hydrophobicity Value	-0.154
Molecular Weight (kDa)	28.4
Isoelectric Point (pI)	6.07

Subcellular localization

Individual Mappings	Localization Confidence PMID Periplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 448 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001804 (387 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3865 Search term: putative periplasmic lysine-, arginine-, ornithine-binding protein

Human Homologs

References

The hisT-purF region of the Escherichia coli K-12 chromosome. Identification of additional genes of the hisT and purF operons. Nonet ML, Marvel CC, Tolan DR J Biol Chem 1987 Sep 5;262(25):12209-17 PubMed ID: 3040734

Molecular characterization and regulation of an operon encoding a system for transport of arginine and ornithine and the ArgR regulatory protein in Pseudomonas aeruginosa. Nishijyo T, Park SM, Lu CD, Itoh Y, Abdelal AT J Bacteriol 1998 Nov;180(21):5559-66 PubMed ID: 9791103

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach. Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F J Biomol Struct Dyn 2020 Sep;38(15):4508-4520 PubMed ID: 31631799