Pseudomonas aeruginosa PAO1, PA3885 (tpbA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3885
Name
tpbA
Replicon chromosome
Genomic location 4350834 - 4351490 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252574.1
GI 15599080
Affymetrix PA3885_at
DNASU PaCD00007691
Entrez 878776
GenBank AAG07272.1
INSDC AAG07272.1
NCBI Locus Tag PA3885
protein_id(GenBank) gb|AAG07272.1|AE004805_10|gnl|PseudoCAP|PA3885
TIGR NTL03PA03885
UniParc UPI00000C5B5A
UniProtKB Acc Q9HXC7
UniProtKB ID Q9HXC7_PSEAE
UniRef100 UniRef100_Q9HXC7
UniRef50 UniRef50_Q9HXC7
UniRef90 UniRef90_Q9HXC7

Product

Feature Type CDS
Coding Frame 1
Product Name
protein tyrosine phosphatase TpbA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 0.59
Kyte-Doolittle Hydrophobicity Value -0.111
Molecular Weight (kDa) 24.0
Isoelectric Point (pI) 7.20

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
cell fractionation evidence
Periplasmic Class 3
Periplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4R0T HYDROLASE 08/01/14 Crystal structure of P. aeruginosa TpbA (C132S) in complex with pTyr Pseudomonas aeruginosa PAO1 2.603 X-RAY DIFFRACTION 99.5
2M3V HYDROLASE 01/26/13 Solution structure of tyrosine phosphatase related to biofilm formation A (TpbA) from Pseudomonas aeruginosa Pseudomonas aeruginosa NOT SOLUTION NMR 97.4
4R0S HYDROLASE 08/01/14 Crystal structure of P. aeruginosa TpbA Pseudomonas aeruginosa PAO1 2.03 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 32 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001786 (406 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3885
Search term: tpbA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Connecting quorum sensing, c-di-GMP, pel polysaccharide, and biofilm formation in Pseudomonas aeruginosa through tyrosine phosphatase TpbA (PA3885).
Ueda A, Wood TK
PLoS Pathog 2009 Jun;5(6):e1000483
PubMed ID: 19543378
Expression, purification, crystallization and preliminary crystallographic analysis of PA3885 (TpbA) from Pseudomonas aeruginosa PAO1.
Yang W, Li K, Bai Y, Zhou R, Zhou W, Bartlam M
Acta Crystallogr Sect F Struct Biol Cryst Commun 2010 Nov 1;66(Pt 11):1473-6
PubMed ID: 21045298