Pseudomonas aeruginosa PAO1, PA3948 (rocA1)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3948
Name	rocA1 Synonym: rocA1
Replicon	chromosome
Genomic location	4428412 - 4429041 (+ strand)
Transposon Mutants	1 transposon mutants in PAO1
Transposon Mutants in orthologs	2 transposon mutants in orthologs
Comment	RocA1 can interact with two sensors, RocS1 (PA3946) and RocS2 (PA3044) and activates transcription of the cupC gene cluster.
Comment	Functional studies in Pseudomonas aeruginosa PAK have shown that overexpression of RocA1 activates transcription of the cupC gene cluster, which leads to the production and assembly of CupC fimbriae on the cell surface and increases biofilm formation. Genetic and bacterial two hybrid analyses have shown that RocA1 can be activated by the RocS1 sensor, as well as the paralogous RocS2 sensor (PA3044).

Cross-References

RefSeq	NP_252637.1
GI	15599143
Affymetrix	PA3948_at
DNASU	PaCD00007724
Entrez	878843
GenBank	AAG07335.1
INSDC	AAG07335.1
NCBI Locus Tag	PA3948
protein_id(GenBank)	gb|AAG07335.1|AE004813_2|gnl|PseudoCAP|PA3948
TIGR	NTL03PA03948
UniParc	UPI00000C5B90
UniProtKB Acc	Q9HX68
UniProtKB ID	Q9HX68_PSEAE
UniRef100	UniRef100_W0YW02
UniRef50	UniRef50_Q4K750
UniRef90	UniRef90_W0YW02

Product

Feature Type	CDS
Coding Frame	1
Product Name	Two-component response regulator RocA1 Product Name Confidence: Class 1
Synonyms	two-component response regulator RocA1
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-0.62
Kyte-Doolittle Hydrophobicity Value	0.175
Molecular Weight (kDa)	23.0
Isoelectric Point (pI)	6.80

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543 Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 618 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001730 (355 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3948 Search term: rocA1 Search term: Two-component response regulator RocA1

Human Homologs

References

Structural and genetic analysis of the bvg locus in Bordetella species. Aricò B, Scarlato V, Monack DM, Falkow S, Rappuoli R Mol Microbiol 1991 Oct;5(10):2481-91 PubMed ID: 1791760

A novel two-component system controls the expression of Pseudomonas aeruginosa fimbrial cup genes. Kulasekara HD, Ventre I, Kulasekara BR, Lazdunski A, Filloux A, Lory S Mol Microbiol 2005 Jan;55(2):368-80 PubMed ID: 15659157

Two-component regulatory systems in Pseudomonas aeruginosa: an intricate network mediating fimbrial and efflux pump gene expression. Sivaneson M, Mikkelsen H, Ventre I, Bordi C, Filloux A Mol Microbiol 2011 Mar;79(5):1353-66 PubMed ID: 21205015