Pseudomonas aeruginosa PAO1, PA3984 (lnt)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3984
Name
lnt
Synonym: cutE
Replicon chromosome
Genomic location 4463904 - 4465439 (+ strand)

Cross-References

RefSeq NP_252673.1
GI 15599179
Affymetrix PA3984_lnt_at
Entrez 878907
GenBank AAG07371.1
INSDC AAG07371.1
NCBI Locus Tag PA3984
protein_id(GenBank) gb|AAG07371.1|AE004816_7|gnl|PseudoCAP|PA3984
TIGR NTL03PA03984
UniParc UPI000012E7A0
UniProtKB Acc Q9ZI86
UniProtKB ID LNT_PSEAE
UniRef100 UniRef100_Q9ZI86
UniRef50 UniRef50_Q9ZI86
UniRef90 UniRef90_Q9ZI86

Product

Feature Type CDS
Coding Frame 1
Product Name
apolipoprotein N-acyltransferase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (56.02 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 7.37
Kyte-Doolittle Hydrophobicity Value 0.364
Molecular Weight (kDa) 56.0
Isoelectric Point (pI) 9.36

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5N6M MEMBRANE PROTEIN 02/15/17 Structure of the membrane integral lipoprotein N-acyltransferase Lnt from P. aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.1 X-RAY DIFFRACTION 100.0
8AQ2 TRANSFERASE 08/11/22 In meso structure of the membrane integral lipoprotein N-acyltransferase Lnt from P. aeruginosa covalently linked with TITC Pseudomonas aeruginosa PAO1 2.6 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 422 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001697 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3984
Search term: lnt

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning and characterization of cutE, a gene involved in copper transport in Escherichia coli.
Rogers SD, Bhave MR, Mercer JF, Camakaris J, Lee BT
J Bacteriol 1991 Nov;173(21):6742-8
PubMed ID: 1938881
Characterization of a temperature-sensitive mutant of Salmonella typhimurium defective in apolipoprotein N-acyltransferase.
Gupta SD, Gan K, Schmid MB, Wu HC
J Biol Chem 1993 Aug 5;268(22):16551-6
PubMed ID: 8344936
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344