Pseudomonas aeruginosa PAO1, PA3987 (leuS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3987
Name
leuS
Replicon chromosome
Genomic location 4466925 - 4469546 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_252676.1
GI 15599182
Affymetrix PA3987_leuS_at
Entrez 878925
GenBank AAG07374.1
INSDC AAG07374.1
NCBI Locus Tag PA3987
protein_id(GenBank) gb|AAG07374.1|AE004816_10|gnl|PseudoCAP|PA3987
TIGR NTL03PA03987
UniParc UPI0000136570
UniProtKB Acc Q9HX33
UniProtKB ID SYL_PSEAE
UniRef100 UniRef100_Q9HX33
UniRef50 UniRef50_Q9HX33
UniRef90 UniRef90_Q9HX33

Product

Feature Type CDS
Coding Frame 1
Product Name
leucyl-tRNA synthetase
Product Name Confidence: Class 2
Synonyms leucine--tRNA ligase
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -19.02
Kyte-Doolittle Hydrophobicity Value -0.332
Molecular Weight (kDa) 97.6
Isoelectric Point (pI) 5.46

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 668 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001694 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3987
Search term: leuS

Human Homologs

Ensembl 110, assembly GRCh38.p14
leucyl-tRNA synthetase 2, mitochondrial [Source:HGNC Symbol;Acc:HGNC:17095]
E-value: 1.7e-159
Percent Identity: 36.4
Ensembl 110, assembly GRCh38.p14
leucyl-tRNA synthetase 2, mitochondrial [Source:HGNC Symbol;Acc:HGNC:17095]
E-value: 1.7e-159
Percent Identity: 36.4
Ensembl 110, assembly GRCh38.p14
leucyl-tRNA synthetase 2, mitochondrial [Source:HGNC Symbol;Acc:HGNC:17095]
E-value: 1.7e-159
Percent Identity: 36.4

References

Molecular cloning and nucleotide sequence of the gene for Escherichia coli leucyl-tRNA synthetase.
Härtlein M, Madern D
Nucleic Acids Res 1987 Dec 23;15(24):10199-210
PubMed ID: 3320963
High-level expression and single-step purification of leucyl-tRNA synthetase from Escherichia coli.
Chen J, Li Y, Wang E, Wang Y
Protein Expr Purif 1999 Feb;15(1):115-20
PubMed ID: 10024478
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994