Pseudomonas aeruginosa PAO1, PA4020 (mpl)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4020
Name
mpl
Replicon chromosome
Genomic location 4498488 - 4499843 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252709.1
GI 15599215
Affymetrix PA4020_mpl_at
Entrez 879000
GenBank AAG07407.1
INSDC AAG07407.1
NCBI Locus Tag PA4020
protein_id(GenBank) gb|AAG07407.1|AE004818_13|gnl|PseudoCAP|PA4020
TIGR NTL03PA04020
UniParc UPI00000C5BC5
UniProtKB Acc Q9HX07
UniProtKB ID Q9HX07_PSEAE
UniRef100 UniRef100_Q9HX07
UniRef50 UniRef50_P37773
UniRef90 UniRef90_Q9HX07

Product

Feature Type CDS
Coding Frame 1
Product Name
UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase
Product Name Confidence: Class 2
Synonyms murein tripeptide ligase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.78
Kyte-Doolittle Hydrophobicity Value -0.002
Molecular Weight (kDa) 48.5
Isoelectric Point (pI) 6.42

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 578 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001663 (497 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4020
Search term: mpl

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Identification of the mpl gene encoding UDP-N-acetylmuramate: L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase in Escherichia coli and its role in recycling of cell wall peptidoglycan.
Mengin-Lecreulx D, van Heijenoort J, Park JT
J Bacteriol 1996 Sep;178(18):5347-52
PubMed ID: 8808921
Identification of novel genes responsible for overexpression of ampC in Pseudomonas aeruginosa PAO1.
Tsutsumi Y, Tomita H, Tanimoto K
Antimicrob Agents Chemother 2013 Dec;57(12):5987-93
PubMed ID: 24041903