Pseudomonas aeruginosa PAO1, PA4049

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4049
Name
Replicon chromosome
Genomic location 4531390 - 4532136 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs
CommentStructural similarity to 2Q2C (Arg/Lys/His-binding protein ArtJ from Geobacillus stearothermophilu, root-mean-square deviation: 1.5 angstroms). Sequence similarity to 3TQL (60%/24%) (ARG). Final predicted ligand: basic amino acid (PMID: 31631799)

Cross-References

RefSeq NP_252738.1
GI 15599244
Affymetrix PA4049_at
Entrez 879073
GenBank AAG07436.1
INSDC AAG07436.1
NCBI Locus Tag PA4049
protein_id(GenBank) gb|AAG07436.1|AE004821_9|gnl|PseudoCAP|PA4049
TIGR NTL03PA04049
UniParc UPI00000C5BDE
UniProtKB Acc Q9HWX9
UniProtKB ID Q9HWX9_PSEAE
UniRef100 UniRef100_Q9HWX9
UniRef50 UniRef50_Q9HWX9
UniRef90 UniRef90_Q9HWX9

Product

Feature Type CDS
Coding Frame 1
Product Name
hypothetical protein
Product Name Confidence: Class 4
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 1.66
Kyte-Doolittle Hydrophobicity Value -0.160
Molecular Weight (kDa) 28.3
Isoelectric Point (pI) 8.17

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Periplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 15 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001635 (1550 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4049

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799