Pseudomonas aeruginosa PAO1, PA4079

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4079
Name
Replicon chromosome
Genomic location 4558302 - 4558991 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
Comment

Cross-References

RefSeq NP_252768.1
GI 15599274
Affymetrix PA4079_at
DNASU PaCD00007784
Entrez 878690
GenBank AAG07466.1
INSDC AAG07466.1
NCBI Locus Tag PA4079
protein_id(GenBank) gb|AAG07466.1|AE004824_1|gnl|PseudoCAP|PA4079
TIGR NTL03PA04079
UniParc UPI00000C5BF9
UniProtKB Acc Q9HWU9
UniProtKB ID Q9HWU9_PSEAE
UniRef100 UniRef100_W0YZG2
UniRef50 UniRef50_W0YZG2
UniRef90 UniRef90_W0YZG2

Product

Feature Type CDS
Coding Frame 1
Product Name
NAD(P)H-dependent carbonyl reductase
Product Name Confidence: Class 1
Synonyms probable dehydrogenase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.34
Kyte-Doolittle Hydrophobicity Value 0.081
Molecular Weight (kDa) 24.3
Isoelectric Point (pI) 5.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 2
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5WQN OXIDOREDUCTASE 11/27/16 Crystal structure of a carbonyl reductase from Pseudomonas aeruginosa PAO1 (condition II) Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2 X-RAY DIFFRACTION 100.0
5WQM OXIDOREDUCTASE 11/27/16 Crystal structure of a carbonyl reductase from Pseudomonas aeruginosa PAO1 (condition I) Pseudomonas aeruginosa PAO1 2.6 X-RAY DIFFRACTION 100.0
5WQO OXIDOREDUCTASE 11/27/16 Crystal structure of a carbonyl reductase from Pseudomonas aeruginosa PAO1 in complex with NADP (condition I) Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.78 X-RAY DIFFRACTION 100.0
5WQP OXIDOREDUCTASE 11/27/16 Crystal structure of a carbonyl reductase from Pseudomonas aeruginosa PAO1 in complex with NADP (condition II) Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.7 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 508 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001607 (450 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4079

Human Homologs

References

Structure and characterization of a NAD(P)H-dependent carbonyl reductase from Pseudomonas aeruginosa PAO1.
Li S, Teng X, Su L, Mao G, Xu Y, Li T, Liu R, Zhang Q, Wang Y, Bartlam M
FEBS Lett 2017 Jun;591(12):1785-1797
PubMed ID: 28524228