Pseudomonas aeruginosa PAO1, PA4119 (aph)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4119
Name
aph
Replicon chromosome
Genomic location 4607578 - 4608384 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252808.1
GI 15599314
Affymetrix PA4119_aph_at
Entrez 880226
GenBank AAG07506.1
INSDC AAG07506.1
NCBI Locus Tag PA4119
protein_id(GenBank) gb|AAG07506.1|AE004828_7|gnl|PseudoCAP|PA4119
TIGR NTL03PA04119
UniParc UPI00000C5C1C
UniProtKB Acc Q9HWR2
UniProtKB ID Q9HWR2_PSEAE
UniRef100 UniRef100_Q9HWR2
UniRef50 UniRef50_B4EF73
UniRef90 UniRef90_Q9HWR2

Product

Feature Type CDS
Coding Frame 1
Product Name
aminoglycoside 3'-phosphotransferase type IIb
Product Name Confidence: Class 1
Synonyms APH3'IIb
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -11.97
Kyte-Doolittle Hydrophobicity Value -0.280
Molecular Weight (kDa) 29.8
Isoelectric Point (pI) 4.89

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
APH(3')-IIb APH(3')-IIb is a chromosomal-encoded aminoglycoside phosphotransferase in P. aeruginosa. [PMID:14638496, PMID:10984043] ARO:3002645 protein homolog model Strict 543.1 99.25 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 63 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001579 (319 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4119
Search term: aph

Human Homologs

References

Sequence and characterization of a novel chromosomal aminoglycoside phosphotransferase gene, aph (3')-IIb, in Pseudomonas aeruginosa.
Hächler H, Santanam P, Kayser FH
Antimicrob Agents Chemother 1996 May;40(5):1254-6
PubMed ID: 8723476