Pseudomonas aeruginosa PAO1, PA4130 (nirA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4130
Name
nirA
Replicon chromosome
Genomic location 4617535 - 4619208 (- strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs

Cross-References

RefSeq NP_252819.1
GI 15599325
Affymetrix PA4130_at
Entrez 881847
GenBank AAG07517.1
INSDC AAG07517.1
NCBI Locus Tag PA4130
protein_id(GenBank) gb|AAG07517.1|AE004829_7|gnl|PseudoCAP|PA4130
TIGR NTL03PA04130
UniParc UPI00000C5C27
UniProtKB Acc Q9HWQ1
UniProtKB ID Q9HWQ1_PSEAE
UniRef100 UniRef100_Q9HWQ1
UniRef50 UniRef50_A9AHG0
UniRef90 UniRef90_A0A022P2R4

Product

Feature Type CDS
Coding Frame 1
Product Name
ferredoxin-dependent nitrite reductase, NirA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.86
Kyte-Doolittle Hydrophobicity Value -0.258
Molecular Weight (kDa) 62.2
Isoelectric Point (pI) 6.24

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 447 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001569 (896 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4130
Search term: nirA

Human Homologs

References

A novel nitrite reductase gene from the cyanobacterium Plectonema boryanum.
Suzuki I, Kikuchi H, Nakanishi S, Fujita Y, Sugiyama T, Omata T
J Bacteriol 1995 Nov;177(21):6137-43
PubMed ID: 7592378
A novel cyanide-inducible gene cluster helps protect Pseudomonas aeruginosa from cyanide.
Frangipani E, Pérez-Martínez I, Williams HD, Cherbuin G, Haas D
Environ Microbiol Rep 2014 Feb;6(1):28-34
PubMed ID: 24596260
NirA Is an Alternative Nitrite Reductase from Pseudomonas aeruginosa with Potential as an Antivirulence Target.
Fenn S, Dubern JF, Cigana C, De Simone M, Lazenby J, Juhas M, Schwager S, Bianconi I, Döring G, Elmsley J, Eberl L, Williams P, Bragonzi A, Cámara M
mBio 2021 Apr 20;12(2)
PubMed ID: 33879591