Pseudomonas aeruginosa PAO1, PA4133

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4133
Name	Synonym: cytN Synonym: ccoN Synonym: fixN
Replicon	chromosome
Genomic location	4622812 - 4624239 (+ strand)
Transposon Mutants	3 transposon mutants in PAO1
Transposon Mutants in orthologs	9 transposon mutants in orthologs

Cross-References

RefSeq	NP_252822.1
GI	15599328
Affymetrix	PA4133_at
Entrez	880051
GenBank	AAG07520.1
INSDC	AAG07520.1
NCBI Locus Tag	PA4133
protein_id(GenBank)	gb|AAG07520.1|AE004830_1|gnl|PseudoCAP|PA4133
TIGR	NTL03PA04133
UniParc	UPI00000C5C2A
UniProtKB Acc	Q9HWP8
UniProtKB ID	Q9HWP8_PSEAE
UniRef100	UniRef100_Q9HWP8
UniRef50	UniRef50_Q9I3G0
UniRef90	UniRef90_A6UZV9

Product

Feature Type	CDS
Coding Frame	1
Product Name	cytochrome c oxidase subunit (cbb3-type) Product Name Confidence: Class 2
Synonyms	cytochrome c oxidase polypeptide I homolog (heme b/copper cytochrome c oxidase subunit)
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	15.00
Kyte-Doolittle Hydrophobicity Value	0.536
Molecular Weight (kDa)	53.3
Isoelectric Point (pI)	9.76

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 267 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001566 (1638 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4133 Search term: cytochrome c oxidase subunit (cbb3-type)

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Genes for a microaerobically induced oxidase complex in Bradyrhizobium japonicum are essential for a nitrogen-fixing endosymbiosis. Preisig O, Anthamatten D, Hennecke H Proc Natl Acad Sci U S A 1993 Apr 15;90(8):3309-13 PubMed ID: 8386371

A homolog of the Rhizobium meliloti nitrogen fixation gene fixN is involved in the production of a microaerobically induced oxidase activity in the phytopathogenic bacterium Agrobacterium tumefaciens. Schlüter A, Rüberg S, Krämer M, Weidner S, Priefer UB Mol Gen Genet 1995 Apr 20;247(2):206-15 PubMed ID: 7753030

Structural and functional analysis of aa3-type and cbb3-type cytochrome c oxidases of Paracoccus denitrificans reveals significant differences in proton-pump design. de Gier JW, Schepper M, Reijnders WN, van Dyck SJ, Slotboom DJ, Warne A, Saraste M, Krab K, Finel M, Stouthamer AH, van Spanning RJ, van der Oost J Mol Microbiol 1996 Jun;20(6):1247-60 PubMed ID: 8809776

A cytochrome cbb3 (cytochrome c) terminal oxidase in Azospirillum brasilense Sp7 supports microaerobic growth. Marchal K, Sun J, Keijers V, Haaker H, Vanderleyden J J Bacteriol 1998 Nov;180(21):5689-96 PubMed ID: 9791120

A novel cyanide-inducible gene cluster helps protect Pseudomonas aeruginosa from cyanide. Frangipani E, Pérez-Martínez I, Williams HD, Cherbuin G, Haas D Environ Microbiol Rep 2014 Feb;6(1):28-34 PubMed ID: 24596260