Pseudomonas aeruginosa PAO1, PA4190 (pqsL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4190
Name
pqsL
Replicon chromosome
Genomic location 4687652 - 4688848 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252879.1
GI 15599385
Affymetrix PA4190_at
Entrez 880329
GenBank AAG07577.1
INSDC AAG07577.1
NCBI Locus Tag PA4190
protein_id(GenBank) gb|AAG07577.1|AE004835_6|gnl|PseudoCAP|PA4190
TIGR NTL03PA04190
UniParc UPI00000C5C63
UniProtKB Acc Q9HWJ1
UniProtKB ID Q9HWJ1_PSEAE
UniRef100 UniRef100_S0IUL0
UniRef50 UniRef50_A0A022NMJ1
UniRef90 UniRef90_A0A022NMJ1

Product

Feature Type CDS
Coding Frame 1
Product Name
probable FAD-dependent monooxygenase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.18
Kyte-Doolittle Hydrophobicity Value -0.154
Molecular Weight (kDa) 44.0
Isoelectric Point (pI) 6.33

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6SW1 ANTIBIOTIC 09/19/19 Crystal Structure of P. aeruginosa PqsL: R41Y, I43R, G45R, C105G mutant Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.8 X-RAY DIFFRACTION 99.0
6FHO OXIDOREDUCTASE 01/15/18 Crystal structure of pqsL, a probable FAD-dependent monooxygenase from Pseudomonas aeruginosa - new refinement Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.75 X-RAY DIFFRACTION 100.0
2X3N OXIDOREDUCTASE 01/25/10 Crystal structure of pqsL, a probable FAD-dependent monooxygenase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.75 X-RAY DIFFRACTION 100.0
6SW2 ANTIBIOTIC 09/19/19 Crystal Structure of P. aeruginosa PqsL in complex with 2-aminobenzoylacetate Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.7 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 308 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001511 (295 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4190
Search term: pqsL

Human Homologs

References

Autolysis and autoaggregation in Pseudomonas aeruginosa colony morphology mutants.
D'Argenio DA, Calfee MW, Rainey PB, Pesci EC
J Bacteriol 2002 Dec;184(23):6481-9
PubMed ID: 12426335
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295