Pseudomonas aeruginosa PAO1, PA4207 (mexI)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4207
Name
mexI
Replicon chromosome
Genomic location 4707535 - 4710624 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_252896.1
GI 15599402
Affymetrix PA4207_at
Entrez 880346
GenBank AAG07594.1
INSDC AAG07594.1
NCBI Locus Tag PA4207
protein_id(GenBank) gb|AAG07594.1|AE004837_5|gnl|PseudoCAP|PA4207
TIGR NTL03PA04207
UniParc UPI00000C5C74
UniProtKB Acc Q9HWH4
UniProtKB ID Q9HWH4_PSEAE
UniRef100 UniRef100_U8NIU1
UniRef50 UniRef50_W0YY82
UniRef90 UniRef90_W0YY82

Product

Feature Type CDS
Coding Frame 1
Product Name
probable Resistance-Nodulation-Cell Division (RND) efflux transporter
Product Name Confidence: Class 1
Synonyms probable RND efflux transporter
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.64
Kyte-Doolittle Hydrophobicity Value 0.358
Molecular Weight (kDa) 111.0
Isoelectric Point (pI) 6.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
MexI MexI is the inner membrane transporter of the efflux complex MexGHI-OpmD. [PMID:15817779, PMID:12917802] ARO:3000808 protein homolog model Perfect 1912.5 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 487 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001500 (799 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4207
Search term: mexI

Human Homologs

References

Characterization of a new efflux pump, MexGHI-OpmD, from Pseudomonas aeruginosa that confers resistance to vanadium.
Aendekerk S, Ghysels B, Cornelis P, Baysse C
Microbiology (Reading) 2002 Aug;148(Pt 8):2371-2381
PubMed ID: 12177331
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa.
Köhler T, Michéa-Hamzehpour M, Henze U, Gotoh N, Curty LK, Pechère JC
Mol Microbiol 1997 Jan;23(2):345-54
PubMed ID: 9044268