Pseudomonas aeruginosa PAO1, PA4217 (phzS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4217
Name
phzS
Replicon chromosome
Genomic location 4720301 - 4721509 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252907.1
GI 15599413
Affymetrix PA4217_at
Entrez 881836
GenBank AAG07605.1
INSDC AAG07605.1
NCBI Locus Tag PA4217
protein_id(GenBank) gb|AAG07605.1|AE004838_9|gnl|PseudoCAP|PA4217
TIGR NTL03PA04218
UniParc UPI00000C5C77
UniProtKB Acc Q9HWG9
UniProtKB ID Q9HWG9_PSEAE
UniRef100 UniRef100_Q9HWG9
UniRef50 UniRef50_Q9HWG9
UniRef90 UniRef90_Q9HWG9

Product

Feature Type CDS
Coding Frame 1
Product Name
flavin-containing monooxygenase
Product Name Confidence: Class 1
Synonyms probable FAD-dependent monooxygenase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.16
Kyte-Doolittle Hydrophobicity Value -0.182
Molecular Weight (kDa) 43.6
Isoelectric Point (pI) 5.94

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3C96 OXIDOREDUCTASE 02/15/08 Crystal structure of the flavin-containing monooxygenase phzS from Pseudomonas aeruginosa. Northeast Structural Genomics Consortium target PaR240 Pseudomonas aeruginosa PAO1 1.9 X-RAY DIFFRACTION 100.0
2RGJ OXIDOREDUCTASE 10/03/07 Crystal structure of flavin-containing monooxygenase PhzS Pseudomonas aeruginosa 2.4 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database PseudoCAP
Category Phenazine biosynthesis
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model chronic pulmonary Infection model
Infection model acute pulmonary Infection model
Reference 15213173
Source 2
Database VFDB : VF0100
Category Phenazine biosynthesis
Evidence
Host Organism
Notes
Reference
Source 3
Database Victors : 4259
Category Phenazine biosynthesis
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Mus musculus
Notes
Reference 15213173

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 221 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001490 (1026 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4217
Search term: phzS

Human Homologs

References

Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1.
Mavrodi DV, Bonsall RF, Delaney SM, Soule MJ, Phillips G, Thomashow LS
J Bacteriol 2001 Nov;183(21):6454-65
PubMed ID: 11591691