Pseudomonas aeruginosa PAO1, PA4226 (pchE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4226
Name
pchE
Replicon chromosome
Genomic location 4736795 - 4741111 (- strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 10 transposon mutants in orthologs

Cross-References

RefSeq NP_252916.1
GI 15599422
Affymetrix PA4226_pchE_at
Entrez 880048
GenBank AAG07614.1
INSDC AAG07614.1
NCBI Locus Tag PA4226
protein_id(GenBank) gb|AAG07614.1|AE004839_7|gnl|PseudoCAP|PA4226
TIGR NTL03PA04227
UniParc UPI00000D421C
UniProtKB Acc G3XCV2
UniProtKB ID G3XCV2_PSEAE
UniRef100 UniRef100_G3XCV2
UniRef50 UniRef50_Q2T477
UniRef90 UniRef90_A0A080VIX7

Product

Feature Type CDS
Coding Frame 1
Product Name
dihydroaeruginoic acid synthetase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -32.03
Kyte-Doolittle Hydrophobicity Value -0.004
Molecular Weight (kDa) 156.4
Isoelectric Point (pI) 5.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7EN2 LIGASE 04/15/21 Pyochelin synthetase, a dimeric nonribosomal peptide synthetase elongation module-after-condensation, condensation Pseudomonas aeruginosa PAO1 3.78 ELECTRON MICROSCOPY 100.0
7EMY LIGASE 04/15/21 Pyochelin synthetase, a dimeric nonribosomal peptide synthetase elongation module Pseudomonas aeruginosa PAO1 2.97 ELECTRON MICROSCOPY 100.0
7EN1 LIGASE 04/15/21 Pyochelin synthetase, a dimeric nonribosomal peptide synthetase elongation module-after-condensation Pseudomonas aeruginosa PAO1 3.47 ELECTRON MICROSCOPY 100.0

Virulence Evidence

Source 1
Database VFDB : VF0095
Category Pyochelin
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 547 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001483 (2117 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4226
Search term: pchE

Human Homologs

Ensembl 110, assembly GRCh38.p14
aminoadipate-semialdehyde dehydrogenase [Source:HGNC Symbol;Acc:HGNC:23993]
E-value: 5.4e-14
Percent Identity: 28.9
Ensembl 110, assembly GRCh38.p14
aminoadipate-semialdehyde dehydrogenase [Source:HGNC Symbol;Acc:HGNC:23993]
E-value: 5.4e-14
Percent Identity: 28.9
Ensembl 110, assembly GRCh38.p14
aminoadipate-semialdehyde dehydrogenase [Source:HGNC Symbol;Acc:HGNC:23993]
E-value: 5.4e-14
Percent Identity: 28.9
Ensembl 110, assembly GRCh38.p14
aminoadipate-semialdehyde dehydrogenase [Source:HGNC Symbol;Acc:HGNC:23993]
E-value: 5.4e-14
Percent Identity: 28.9
Ensembl 110, assembly GRCh38.p14
aminoadipate-semialdehyde dehydrogenase [Source:HGNC Symbol;Acc:HGNC:23993]
E-value: 5.4e-14
Percent Identity: 28.9

References

Dihydroaeruginoic acid synthetase and pyochelin synthetase, products of the pchEF genes, are induced by extracellular pyochelin in Pseudomonas aeruginosa.
Reimmann C, Serino L, Beyeler M, Haa D
Microbiology (Reading) 1998 Nov;144 ( Pt 11):3135-3148
PubMed ID: 9846750