Pseudomonas aeruginosa PAO1, PA4228 (pchD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4228
Name
pchD
Replicon chromosome
Genomic location 4742424 - 4744067 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252918.1
GI 15599424
Affymetrix PA4228_pchD_at
Entrez 880566
GenBank AAG07616.1
INSDC AAG07616.1
NCBI Locus Tag PA4228
protein_id(GenBank) gb|AAG07616.1|AE004840_2|gnl|PseudoCAP|PA4228
TIGR NTL03PA04229
UniParc UPI00000C5C7B
UniProtKB Acc Q9HWG3
UniProtKB ID Q9HWG3_PSEAE
UniRef100 UniRef100_Q9HWG3
UniRef50 UniRef50_O07899
UniRef90 UniRef90_A0A0A8RA39

Product

Feature Type CDS
Coding Frame 1
Product Name
pyochelin biosynthesis protein PchD
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -1.34
Kyte-Doolittle Hydrophobicity Value -0.185
Molecular Weight (kDa) 59.9
Isoelectric Point (pI) 6.85

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7TZ4 BIOSYNTHETIC PROTEIN 02/15/22 Salicylate Adenylate PchD from Pseudomonas aeruginosa containing 4-cyanosalicyl-AMS Pseudomonas aeruginosa 1.69 X-RAY DIFFRACTION 100.0
7TYB BIOSYNTHETIC PROTEIN 02/11/22 Salicylate Adenylate PchD from Pseudomonas aeruginosa containing salicyl-AMS Pseudomonas aeruginosa PAO1 2.11 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0095
Category Pyochelin
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 604 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001481 (356 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4228
Search term: pchD

Human Homologs

References

Structural genes for salicylate biosynthesis from chorismate in Pseudomonas aeruginosa.
Serino L, Reimmann C, Baur H, Beyeler M, Visca P, Haas D
Mol Gen Genet 1995 Nov 15;249(2):217-28
PubMed ID: 7500944