Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4232
|
Name |
ssb
|
Replicon | chromosome |
Genomic location | 4746640 - 4747137 (- strand) |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_252922.1 |
GI | 15599428 |
Affymetrix | PA4232_ssb_at |
Entrez | 881822 |
GenBank | AAG07620.1 |
INSDC | AAG07620.1 |
NCBI Locus Tag | PA4232 |
protein_id(GenBank) | gb|AAG07620.1|AE004840_6|gnl|PseudoCAP|PA4232 |
TIGR | NTL03PA04233 |
UniParc | UPI0000135FB0 |
UniProtKB Acc | P40947 |
UniProtKB ID | SSB_PSEAE |
UniRef100 | UniRef100_P40947 |
UniRef50 | UniRef50_P40947 |
UniRef90 | UniRef90_P40947 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
single-stranded DNA-binding protein
|
Synonyms | |
Evidence for Translation |
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.5, molecular weight:18.5, PMID:21674802)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -1.77 |
Kyte-Doolittle Hydrophobicity Value | -1.028 |
Molecular Weight (kDa) | 18.6 |
Isoelectric Point (pI) | 5.28 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6JDG | DNA BINDING PROTEIN/DNA | 02/01/19 | Complexed crystal structure of PaSSB with ssDNA dT20 at 2.39 angstrom resolution | Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.388 | X-RAY DIFFRACTION | 100.0 |
5YUO | DNA BINDING PROTEIN | 11/22/17 | Crystal structure of SSB protein from Pseudomonas aeruginosa PAO1 | Pseudomonas aeruginosa PAO1 | 2.037 | X-RAY DIFFRACTION | 100.0 |
6IRQ | DNA BINDING PROTEIN/DNA | 11/14/18 | Complexed crystal structure of PaSSB with ssDNA dT25 at 1.91 angstrom resolution | Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 1.91 | X-RAY DIFFRACTION | 100.0 |
5YUN | DNA BINDING PROTEIN | 11/22/17 | Crystal structure of SSB complexed with myc | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 2.67 | X-RAY DIFFRACTION | 100.0 |
7VUM | DNA BINDING PROTEIN | 11/03/21 | Crystal structure of SSB complexed with que | Pseudomonas aeruginosa PAO1 | 2.319 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 586 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001478 (538 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4232
Search term: ssb
Search term: single-stranded DNA-binding protein
|
Ensembl
110, assembly
GRCh38.p14
|
single stranded DNA binding protein 1 [Source:HGNC Symbol;Acc:HGNC:11317]
E-value:
2.6e-13
Percent Identity:
35.5
|
Ensembl
110, assembly
GRCh38.p14
|
single stranded DNA binding protein 1 [Source:HGNC Symbol;Acc:HGNC:11317]
E-value:
2.6e-13
Percent Identity:
35.5
|
Ensembl
110, assembly
GRCh38.p14
|
single stranded DNA binding protein 1 [Source:HGNC Symbol;Acc:HGNC:11317]
E-value:
2.6e-13
Percent Identity:
35.5
|
Ensembl
110, assembly
GRCh38.p14
|
single stranded DNA binding protein 1 [Source:HGNC Symbol;Acc:HGNC:11317]
E-value:
2.6e-13
Percent Identity:
35.5
|
Ensembl
110, assembly
GRCh38.p14
|
single stranded DNA binding protein 1 [Source:HGNC Symbol;Acc:HGNC:11317]
E-value:
2.6e-13
Percent Identity:
35.5
|
Ensembl
110, assembly
GRCh38.p14
|
single stranded DNA binding protein 1 [Source:HGNC Symbol;Acc:HGNC:11317]
E-value:
2.6e-13
Percent Identity:
35.5
|
Ensembl
110, assembly
GRCh38.p14
|
single stranded DNA binding protein 1 [Source:HGNC Symbol;Acc:HGNC:11317]
E-value:
2.6e-13
Percent Identity:
35.5
|
Isolation, sequencing and overproduction of the single-stranded DNA binding protein from Pseudomonas aeruginosa PAO.
Genschel J, Litz L, Thole H, Roemling U, Urbanke C
Gene 1996 Dec 5;182(1-2):137-43
PubMed ID: 8982079
|
Characterization of a single-stranded DNA-binding protein from Pseudomonas aeruginosa PAO1.
Jan HC, Lee YL, Huang CY
Protein J 2011 Jan;30(1):20-6
PubMed ID: 21132356
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802
|