Pseudomonas aeruginosa PAO1, PA4232 (ssb)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4232
Name
ssb
Replicon chromosome
Genomic location 4746640 - 4747137 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252922.1
GI 15599428
Affymetrix PA4232_ssb_at
Entrez 881822
GenBank AAG07620.1
INSDC AAG07620.1
NCBI Locus Tag PA4232
protein_id(GenBank) gb|AAG07620.1|AE004840_6|gnl|PseudoCAP|PA4232
TIGR NTL03PA04233
UniParc UPI0000135FB0
UniProtKB Acc P40947
UniProtKB ID SSB_PSEAE
UniRef100 UniRef100_P40947
UniRef50 UniRef50_P40947
UniRef90 UniRef90_P40947

Product

Feature Type CDS
Coding Frame 1
Product Name
single-stranded DNA-binding protein
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.5, molecular weight:18.5, PMID:21674802)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.77
Kyte-Doolittle Hydrophobicity Value -1.028
Molecular Weight (kDa) 18.6
Isoelectric Point (pI) 5.28

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6JDG DNA BINDING PROTEIN/DNA 02/01/19 Complexed crystal structure of PaSSB with ssDNA dT20 at 2.39 angstrom resolution Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.388 X-RAY DIFFRACTION 100.0
5YUO DNA BINDING PROTEIN 11/22/17 Crystal structure of SSB protein from Pseudomonas aeruginosa PAO1 Pseudomonas aeruginosa PAO1 2.037 X-RAY DIFFRACTION 100.0
6IRQ DNA BINDING PROTEIN/DNA 11/14/18 Complexed crystal structure of PaSSB with ssDNA dT25 at 1.91 angstrom resolution Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 1.91 X-RAY DIFFRACTION 100.0
5YUN DNA BINDING PROTEIN 11/22/17 Crystal structure of SSB complexed with myc Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.67 X-RAY DIFFRACTION 100.0
7VUM DNA BINDING PROTEIN 11/03/21 Crystal structure of SSB complexed with que Pseudomonas aeruginosa PAO1 2.319 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 586 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001478 (538 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4232
Search term: ssb

Human Homologs

Ensembl 110, assembly GRCh38.p14
single stranded DNA binding protein 1 [Source:HGNC Symbol;Acc:HGNC:11317]
E-value: 2.6e-13
Percent Identity: 35.5
Ensembl 110, assembly GRCh38.p14
single stranded DNA binding protein 1 [Source:HGNC Symbol;Acc:HGNC:11317]
E-value: 2.6e-13
Percent Identity: 35.5
Ensembl 110, assembly GRCh38.p14
single stranded DNA binding protein 1 [Source:HGNC Symbol;Acc:HGNC:11317]
E-value: 2.6e-13
Percent Identity: 35.5
Ensembl 110, assembly GRCh38.p14
single stranded DNA binding protein 1 [Source:HGNC Symbol;Acc:HGNC:11317]
E-value: 2.6e-13
Percent Identity: 35.5
Ensembl 110, assembly GRCh38.p14
single stranded DNA binding protein 1 [Source:HGNC Symbol;Acc:HGNC:11317]
E-value: 2.6e-13
Percent Identity: 35.5
Ensembl 110, assembly GRCh38.p14
single stranded DNA binding protein 1 [Source:HGNC Symbol;Acc:HGNC:11317]
E-value: 2.6e-13
Percent Identity: 35.5
Ensembl 110, assembly GRCh38.p14
single stranded DNA binding protein 1 [Source:HGNC Symbol;Acc:HGNC:11317]
E-value: 2.6e-13
Percent Identity: 35.5

References

Isolation, sequencing and overproduction of the single-stranded DNA binding protein from Pseudomonas aeruginosa PAO.
Genschel J, Litz L, Thole H, Roemling U, Urbanke C
Gene 1996 Dec 5;182(1-2):137-43
PubMed ID: 8982079
Characterization of a single-stranded DNA-binding protein from Pseudomonas aeruginosa PAO1.
Jan HC, Lee YL, Huang CY
Protein J 2011 Jan;30(1):20-6
PubMed ID: 21132356
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802