Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4246
|
Name |
rpsE
|
Replicon | chromosome |
Genomic location | 4759070 - 4759570 (- strand) |
Transposon Mutants | 1 transposon mutants in PAO1 |
RefSeq | NP_252936.1 |
GI | 15599442 |
Affymetrix | PA4246_rpsE_at |
Entrez | 881788 |
GenBank | AAG07634.1 |
INSDC | AAG07634.1 |
NCBI Locus Tag | PA4246 |
protein_id(GenBank) | gb|AAG07634.1|AE004841_12|gnl|PseudoCAP|PA4246 |
TIGR | NTL03PA04247 |
UniParc | UPI0000135068 |
UniProtKB Acc | Q9HWF2 |
UniProtKB ID | RS5_PSEAE |
UniRef100 | UniRef100_A6UZK5 |
UniRef50 | UniRef50_A3M967 |
UniRef90 | UniRef90_Q889V4 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
30S ribosomal protein S5
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected using LC-MS/MS (17.63 kDa, PMID:21751344)
|
Charge (pH 7) | 11.19 |
Kyte-Doolittle Hydrophobicity Value | -0.193 |
Molecular Weight (kDa) | 17.6 |
Isoelectric Point (pI) | 10.57 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
7UNU | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.9 | ELECTRON MICROSCOPY | 100.0 |
7UNR | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.9 | ELECTRON MICROSCOPY | 100.0 |
6SPE | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 30s ribosome from a clinical isolate | Pseudomonas aeruginosa | 3.6 | ELECTRON MICROSCOPY | 100.0 |
6SPG | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 70s ribosome from a clinical isolate | Pseudomonas aeruginosa | 3.34 | ELECTRON MICROSCOPY | 100.0 |
6SPF | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate | Pseudomonas aeruginosa | 2.89 | ELECTRON MICROSCOPY | 100.0 |
7UNV | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.7 | ELECTRON MICROSCOPY | 100.0 |
7UNW | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.6 | ELECTRON MICROSCOPY | 100.0 |
6SPC | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 30s ribosome from an aminoglycoside resistant clinical isolate | Pseudomonas aeruginosa | 2.95 | ELECTRON MICROSCOPY | 99.3 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001464 (535 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4246
Search term: rpsE
Search term: 30S ribosomal protein S5
|
The primary structure of protein S5 from the small subunit of the Escherichia coli ribosome.
Wittmann-Liebold B, Greuer B
FEBS Lett 1978 Nov 1;95(1):91-8
PubMed ID: 363452
|
The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene.
Cerretti DP, Dean D, Davis GR, Bedwell DM, Nomura M
Nucleic Acids Res 1983 May 11;11(9):2599-616
PubMed ID: 6222285
|
Cloning and nucleotide sequencing of the genes rimI and rimJ which encode enzymes acetylating ribosomal proteins S18 and S5 of Escherichia coli K12.
Yoshikawa A, Isono S, Sheback A, Isono K
Mol Gen Genet 1987 Oct;209(3):481-8
PubMed ID: 2828880
|
Ribosomal proteins S5 and L6: high-resolution crystal structures and roles in protein synthesis and antibiotic resistance.
Davies C, Bussiere DE, Golden BL, Porter SJ, Ramakrishnan V, White SW
J Mol Biol 1998 Jun 19;279(4):873-88
PubMed ID: 9642068
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|