Pseudomonas aeruginosa PAO1, PA4248 (rplF)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4248
Name	rplF
Replicon	chromosome
Genomic location	4759935 - 4760468 (- strand)

Cross-References

RefSeq	NP_252938.1
GI	15599444
Affymetrix	PA4248_rplF_at
Entrez	881796
GenBank	AAG07636.1
INSDC	AAG07636.1
NCBI Locus Tag	PA4248
protein_id(GenBank)	gb|AAG07636.1|AE004841_14|gnl|PseudoCAP|PA4248
TIGR	NTL03PA04249
UniParc	UPI00000C5C84
UniProtKB Acc	Q9HWF0
UniProtKB ID	RL6_PSEAE
UniRef100	UniRef100_A6UZK3
UniRef50	UniRef50_A4VHP5
UniRef90	UniRef90_A4VHP5

Product

Feature Type	CDS
Coding Frame	1
Product Name	50S ribosomal protein L6 Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Detected by two-dimensional gel electrophoresis and protein mass mapping(PMID:21863142). Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Charge (pH 7)	10.47
Kyte-Doolittle Hydrophobicity Value	-0.296
Molecular Weight (kDa)	19.1
Isoelectric Point (pI)	10.53

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6SPB	RIBOSOME	09/01/19	Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6	Pseudomonas aeruginosa	2.82	ELECTRON MICROSCOPY	97.7
7UNW	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.6	ELECTRON MICROSCOPY	100.0
6SPF	RIBOSOME	09/01/19	Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate	Pseudomonas aeruginosa	2.89	ELECTRON MICROSCOPY	97.7
6SPD	RIBOSOME	09/01/19	Pseudomonas aeruginosa 50s ribosome from a clinical isolate	Pseudomonas aeruginosa	3.28	ELECTRON MICROSCOPY	100.0
7UNU	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
7UNV	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.7	ELECTRON MICROSCOPY	100.0
6SPG	RIBOSOME	09/01/19	Pseudomonas aeruginosa 70s ribosome from a clinical isolate	Pseudomonas aeruginosa	3.34	ELECTRON MICROSCOPY	100.0
7UNR	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 642 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001462 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4248 Search term: rplF Search term: 50S ribosomal protein L6

Human Homologs

References

The primary structure of protein L6 from the aminoacyl-tRNA binding site of the Escherichia coli ribosome. Chen R, Arfsten U, Chen-Schmeisser U Hoppe Seylers Z Physiol Chem 1977 Apr;358(4):531-5 PubMed ID: 324885

The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene. Cerretti DP, Dean D, Davis GR, Bedwell DM, Nomura M Nucleic Acids Res 1983 May 11;11(9):2599-616 PubMed ID: 6222285

Ribosomal proteins S5 and L6: high-resolution crystal structures and roles in protein synthesis and antibiotic resistance. Davies C, Bussiere DE, Golden BL, Porter SJ, Ramakrishnan V, White SW J Mol Biol 1998 Jun 19;279(4):873-88 PubMed ID: 9642068

Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient. Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB Front Microbiol 2011;2:162 PubMed ID: 21863142