Pseudomonas aeruginosa PAO1, PA4251 (rplE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4251
Name
rplE
Replicon chromosome
Genomic location 4761381 - 4761920 (- strand)

Cross-References

RefSeq NP_252941.1
GI 15599447
Affymetrix PA4251_rplE_at
DNASU PaCD00007846
Entrez 881779
GenBank AAG07639.1
INSDC AAG07639.1
NCBI Locus Tag PA4251
protein_id(GenBank) gb|AAG07639.1|AE004841_17|gnl|PseudoCAP|PA4251
TIGR NTL03PA04252
UniParc UPI00000C5C85
UniProtKB Acc Q9HWE7
UniProtKB ID RL5_PSEAE
UniRef100 UniRef100_B7V656
UniRef50 UniRef50_P08895
UniRef90 UniRef90_Q4K545

Product

Feature Type CDS
Coding Frame 1
Product Name
50S ribosomal protein L5
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (20.39 kDa, PMID:21751344)
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 8.00
Kyte-Doolittle Hydrophobicity Value -0.313
Molecular Weight (kDa) 20.4
Isoelectric Point (pI) 10.30

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6SPB RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6 Pseudomonas aeruginosa 2.82 ELECTRON MICROSCOPY 98.3
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPD RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate Pseudomonas aeruginosa 3.28 ELECTRON MICROSCOPY 99.4
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 99.4
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 99.4
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 665 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001459 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4251
Search term: rplE

Human Homologs

References

The primary structure of the 5 S RNA binding protein L5 of Escherichia coli ribosomes.
Chen R, Ehrke G
FEBS Lett 1976 Oct 15;69(1):240-5
PubMed ID: 791672
The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene.
Cerretti DP, Dean D, Davis GR, Bedwell DM, Nomura M
Nucleic Acids Res 1983 May 11;11(9):2599-616
PubMed ID: 6222285
Nuclease protection analysis of ribonucleoprotein complexes: use of the cytotoxic ribonuclease alpha-sarcin to determine the binding sites for Escherichia coli ribosomal proteins L5, L18, and L25 on 5S rRNA.
Huber PW, Wool IG
Proc Natl Acad Sci U S A 1984 Jan;81(2):322-6
PubMed ID: 6364140
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344