Pseudomonas aeruginosa PAO1, PA4255 (rpmC)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4255
Name	rpmC
Replicon	chromosome
Genomic location	4762928 - 4763119 (- strand)

Cross-References

RefSeq	NP_252945.1
GI	15599451
Affymetrix	PA4255_rpmC_at
Entrez	881771
GenBank	AAG07643.1
INSDC	AAG07643.1
NCBI Locus Tag	PA4255
protein_id(GenBank)	gb|AAG07643.1|AE004841_21|gnl|PseudoCAP|PA4255
TIGR	NTL03PA04256
UniParc	UPI0000133E83
UniProtKB Acc	Q9HWE3
UniProtKB ID	RL29_PSEAE
UniRef100	UniRef100_A6UZJ6
UniRef50	UniRef50_Q6CZX8
UniRef90	UniRef90_A4VHN8

Product

Feature Type	CDS
Coding Frame	1
Product Name	50S ribosomal protein L29 Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	5.22
Kyte-Doolittle Hydrophobicity Value	-0.700
Molecular Weight (kDa)	7.2
Isoelectric Point (pI)	11.05

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543 Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
7UNU	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
7UNW	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.6	ELECTRON MICROSCOPY	100.0
7UNR	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
7UNV	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.7	ELECTRON MICROSCOPY	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 383 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001455 (533 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4255 Search term: rpmC Search term: 50S ribosomal protein L29

Human Homologs

References

The primary structure of the ribosomal protein L29 from Escherichia coli. Bitar KG Biochim Biophys Acta 1975 Mar 28;386(1):99-106 PubMed ID: 1092361

Structure of the Escherichia coli S10 ribosomal protein operon. Zurawski G, Zurawski SM Nucleic Acids Res 1985 Jun 25;13(12):4521-6 PubMed ID: 3892488

A mutant from Escherichia coli which lacks ribosomal proteins S17 and L29 used to localize these two proteins on the ribosomal surface. Stöffler-Meilicke M, Dabbs ER, Albrecht-Ehrlich R, Stöffler G Eur J Biochem 1985 Aug 1;150(3):485-90 PubMed ID: 3926498

Host proteins can stimulate Tn7 transposition: a novel role for the ribosomal protein L29 and the acyl carrier protein. Sharpe PL, Craig NL EMBO J 1998 Oct 1;17(19):5822-31 PubMed ID: 9755182