Pseudomonas aeruginosa PAO1, PA4256 (rplP)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4256
Name
rplP
Replicon chromosome
Genomic location 4763119 - 4763532 (- strand)

Cross-References

RefSeq NP_252946.1
GI 15599452
Affymetrix PA4256_rplP_at
Entrez 881758
GenBank AAG07644.1
INSDC AAG07644.1
NCBI Locus Tag PA4256
protein_id(GenBank) gb|AAG07644.1|AE004841_22|gnl|PseudoCAP|PA4256
TIGR NTL03PA04257
UniParc UPI00000C5C89
UniProtKB Acc Q9HWE2
UniProtKB ID RL16_PSEAE
UniRef100 UniRef100_B7V651
UniRef50 UniRef50_Q9CL38
UniRef90 UniRef90_C1DKM0

Product

Feature Type CDS
Coding Frame 1
Product Name
50S ribosomal protein L16
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 20.70
Kyte-Doolittle Hydrophobicity Value -0.476
Molecular Weight (kDa) 15.4
Isoelectric Point (pI) 11.79

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 100.0
6SPD RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate Pseudomonas aeruginosa 3.28 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0
6SPB RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6 Pseudomonas aeruginosa 2.82 ELECTRON MICROSCOPY 100.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 592 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001454 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4256
Search term: rplP

Human Homologs

References

DNA sequence of the promoter region for the alpha ribosomal protein operon in Escherichia coli.
Post LE, Arfsten AE, Davis GR, Nomura M
J Biol Chem 1980 May 25;255(10):4653-59
PubMed ID: 6154696
Structure of the Escherichia coli S10 ribosomal protein operon.
Zurawski G, Zurawski SM
Nucleic Acids Res 1985 Jun 25;13(12):4521-6
PubMed ID: 3892488
The peptidyltransferase centre of the Escherichia coli ribosome. The histidine of protein L16 affects the reconstitution and control of the active centre but is not essential for release-factor-mediated peptidyl-tRNA hydrolysis and peptide bond formation.
Tate WP, Sumpter VG, Trotman CN, Herold M, Nierhaus KH
Eur J Biochem 1987 Jun 1;165(2):403-8
PubMed ID: 3297687
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142