Pseudomonas aeruginosa PAO1, PA4258 (rplV)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4258
Name	rplV
Replicon	chromosome
Genomic location	4764243 - 4764575 (- strand)

Cross-References

RefSeq	NP_252948.1
GI	15599454
Affymetrix	PA4258_rplV_at
Entrez	881762
GenBank	AAG07646.1
INSDC	AAG07646.1
NCBI Locus Tag	PA4258
protein_id(GenBank)	gb|AAG07646.1|AE004841_24|gnl|PseudoCAP|PA4258
TIGR	NTL03PA04259
UniParc	UPI000000EB39
UniProtKB Acc	Q9HWE0
UniProtKB ID	RL22_PSEAE
UniRef100	UniRef100_A6UZJ3
UniRef50	UniRef50_B2HZL7
UniRef90	UniRef90_A4VHN5

Product

Feature Type	CDS
Coding Frame	1
Product Name	50S ribosomal protein L22 Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	10.44
Kyte-Doolittle Hydrophobicity Value	-0.251
Molecular Weight (kDa)	11.9
Isoelectric Point (pI)	10.90

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543 Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6SPF	RIBOSOME	09/01/19	Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate	Pseudomonas aeruginosa	2.89	ELECTRON MICROSCOPY	100.0
7UNU	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
6SPD	RIBOSOME	09/01/19	Pseudomonas aeruginosa 50s ribosome from a clinical isolate	Pseudomonas aeruginosa	3.28	ELECTRON MICROSCOPY	100.0
7UNV	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.7	ELECTRON MICROSCOPY	100.0
6SPB	RIBOSOME	09/01/19	Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6	Pseudomonas aeruginosa	2.82	ELECTRON MICROSCOPY	100.0
7UNR	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
7UNW	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.6	ELECTRON MICROSCOPY	100.0
6SPG	RIBOSOME	09/01/19	Pseudomonas aeruginosa 70s ribosome from a clinical isolate	Pseudomonas aeruginosa	3.34	ELECTRON MICROSCOPY	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 612 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001452 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4258 Search term: rplV Search term: 50S ribosomal protein L22

Human Homologs

References

Amino acid sequence of protein L22 from the large subunit of the Escherichia coli ribosome. Wittmann-Liebold B, Greuer B FEBS Lett 1980 Nov 17;121(1):105-12 PubMed ID: 7007072

Structure of the Escherichia coli S10 ribosomal protein operon. Zurawski G, Zurawski SM Nucleic Acids Res 1985 Jun 25;13(12):4521-6 PubMed ID: 3892488

A temperature-sensitive mutant of Escherichia coli with an alteration in ribosomal protein L22. Burnette-Vick B, Champney WS, Musich PR Genetica 1994;94(1):17-25 PubMed ID: 7729693