Pseudomonas aeruginosa PAO1, PA4260 (rplB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4260
Name
rplB
Replicon chromosome
Genomic location 4764880 - 4765701 (- strand)

Cross-References

RefSeq NP_252950.1
GI 15599456
Affymetrix PA4260_rplB_at
DNASU PaCD00007849
Entrez 881749
GenBank AAG07648.1
INSDC AAG07648.1
NCBI Locus Tag PA4260
protein_id(GenBank) gb|AAG07648.1|AE004841_26|gnl|PseudoCAP|PA4260
TIGR NTL03PA04261
UniParc UPI00000C5C8A
UniProtKB Acc Q9HWD8
UniProtKB ID RL2_PSEAE
UniRef100 UniRef100_A6UZJ1
UniRef50 UniRef50_Q85FI1
UniRef90 UniRef90_Q889W8

Product

Feature Type CDS
Coding Frame 1
Product Name
50S ribosomal protein L2
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 35.33
Kyte-Doolittle Hydrophobicity Value -0.604
Molecular Weight (kDa) 29.6
Isoelectric Point (pI) 11.79

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPB RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6 Pseudomonas aeruginosa 2.82 ELECTRON MICROSCOPY 100.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
6SPD RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate Pseudomonas aeruginosa 3.28 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 668 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001450 (525 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4260
Search term: rplB

Human Homologs

Ensembl 110, assembly GRCh38.p14
ribosomal protein L8 [Source:HGNC Symbol;Acc:HGNC:10368]
E-value: 3.2e-16
Percent Identity: 31.4
Ensembl 110, assembly GRCh38.p14
ribosomal protein L8 [Source:HGNC Symbol;Acc:HGNC:10368]
E-value: 3.2e-16
Percent Identity: 31.4
Ensembl 110, assembly GRCh38.p14
ribosomal protein L8 [Source:HGNC Symbol;Acc:HGNC:10368]
E-value: 3.2e-16
Percent Identity: 31.4

References

Structure of the Escherichia coli S10 ribosomal protein operon.
Zurawski G, Zurawski SM
Nucleic Acids Res 1985 Jun 25;13(12):4521-6
PubMed ID: 3892488
Functional implications of ribosomal protein L2 in protein biosynthesis as shown by in vivo replacement studies.
Uhlein M, Weglöhner W, Urlaub H, Wittmann-Liebold B
Biochem J 1998 Apr 15;331 ( Pt 2)(Pt 2):423-30
PubMed ID: 9531480