Pseudomonas aeruginosa PAO1, PA4263 (rplC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4263
Name
rplC
Replicon chromosome
Genomic location 4766625 - 4767260 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_252953.1
GI 15599459
Affymetrix PA4263_rplC_at
Entrez 881728
GenBank AAG07651.1
INSDC AAG07651.1
NCBI Locus Tag PA4263
protein_id(GenBank) gb|AAG07651.1|AE004841_29|gnl|PseudoCAP|PA4263
TIGR NTL03PA04264
UniParc UPI00000C5C8D
UniProtKB Acc Q9HWD5
UniProtKB ID RL3_PSEAE
UniRef100 UniRef100_A6UZI8
UniRef50 UniRef50_A2SLF7
UniRef90 UniRef90_Q889X1

Product

Feature Type CDS
Coding Frame 1
Product Name
50S ribosomal protein L3
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 9.90
Kyte-Doolittle Hydrophobicity Value -0.329
Molecular Weight (kDa) 22.6
Isoelectric Point (pI) 10.55

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 100.0
6SPB RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6 Pseudomonas aeruginosa 2.82 ELECTRON MICROSCOPY 100.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPD RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate Pseudomonas aeruginosa 3.28 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 620 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001447 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4263
Search term: rplC

Human Homologs

Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein L3 [Source:HGNC Symbol;Acc:HGNC:10379]
E-value: 1.1e-21
Percent Identity: 31.1
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein L3 [Source:HGNC Symbol;Acc:HGNC:10379]
E-value: 1.1e-21
Percent Identity: 31.1
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein L3 [Source:HGNC Symbol;Acc:HGNC:10379]
E-value: 1.1e-21
Percent Identity: 31.1

References

Regulation of the S10 ribosomal protein operon in E. coli: nucleotide sequence at the start of the operon.
Olins PO, Nomura M
Cell 1981 Oct;26(2 Pt 2):205-11
PubMed ID: 7037196
Structure of the Escherichia coli S10 ribosomal protein operon.
Zurawski G, Zurawski SM
Nucleic Acids Res 1985 Jun 25;13(12):4521-6
PubMed ID: 3892488
Interaction of the sarcin/ricin domain of 23 S ribosomal RNA with proteins L3 and L6.
Uchiumi T, Sato N, Wada A, Hachimori A
J Biol Chem 1999 Jan 8;274(2):681-6
PubMed ID: 9873002
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142