Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4264
|
Name |
rpsJ
|
Replicon | chromosome |
Genomic location | 4767343 - 4767654 (- strand) |
RefSeq | NP_252954.1 |
GI | 15599460 |
Affymetrix | PA4264_rpsJ_at |
DNASU | PaCD00007851 |
Entrez | 881717 |
GenBank | AAG07652.1 |
INSDC | AAG07652.1 |
NCBI Locus Tag | PA4264 |
protein_id(GenBank) | gb|AAG07652.1|AE004842_1|gnl|PseudoCAP|PA4264 |
TIGR | NTL03PA04265 |
UniParc | UPI0000134BBD |
UniProtKB Acc | Q9HWD4 |
UniProtKB ID | RS10_PSEAE |
UniRef100 | UniRef100_A6UZI7 |
UniRef50 | UniRef50_P19460 |
UniRef90 | UniRef90_B4RZN1 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
30S ribosomal protein S10
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
|
Charge (pH 7) | 4.71 |
Kyte-Doolittle Hydrophobicity Value | -0.488 |
Molecular Weight (kDa) | 11.8 |
Isoelectric Point (pI) | 10.47 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
7N1P | RIBOSOME | 05/28/21 | Elongating 70S ribosome complex in a classical pre-translocation (PRE-C) conformation | Escherichia coli (strain K12); Escherichia coli K-12; SYNTHETIC CONSTRUCT | 2.33 | ELECTRON MICROSCOPY | 90.3 |
4V56 | RIBOSOME | 07/21/07 | Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. | Escherichia coli | 3.93 | X-RAY DIFFRACTION | 90.3 |
7UNU | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.9 | ELECTRON MICROSCOPY | 100.0 |
6SPC | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 30s ribosome from an aminoglycoside resistant clinical isolate | Pseudomonas aeruginosa | 2.95 | ELECTRON MICROSCOPY | 100.0 |
4V5B | RIBOSOME | 11/22/07 | Structure of PDF binding helix in complex with the ribosome. | ESCHERICHIA COLI; SYNTHETIC CONSTRUCT | 3.74 | X-RAY DIFFRACTION | 90.3 |
4V6C | RIBOSOME | 06/27/09 | Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting | Escherichia coli; Escherichia coli K-12 | 3.19 | X-RAY DIFFRACTION | 90.3 |
7NAV | RIBOSOME | 01/25/21 | Bacterial 30S ribosomal subunit assembly complex state D (Consensus refinement) | Escherichia coli (strain K12) | 4.8 | ELECTRON MICROSCOPY | 90.3 |
7UNW | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.6 | ELECTRON MICROSCOPY | 100.0 |
7NWT | RIBOSOME | 03/17/21 | Initiated 70S ribosome in complex with 2A protein from encephalomyocarditis virus (EMCV) | Escherichia coli; Mengo encephalomyocarditis virus; SYNTHETIC CONSTRUCT | 2.66 | ELECTRON MICROSCOPY | 90.3 |
7OE0 | RIBOSOME | 04/30/21 | E. coli pre-30S delta rbfA ribosomal subunit class F | Escherichia coli BW25113 | 2.69 | ELECTRON MICROSCOPY | 90.3 |
7O19 | TRANSLATION | 03/29/21 | Cryo-EM structure of an Escherichia coli TnaC-ribosome complex stalled in response to L-tryptophan | Escherichia coli K-12; SYNTHETIC CONSTRUCT | 2.9 | ELECTRON MICROSCOPY | 90.3 |
4WOI | RIBOSOME | 10/15/14 | 4,5-linked aminoglycoside antibiotics regulate the bacterial ribosome by targeting dynamic conformational processes within intersubunit bridge B2 | Escherichia coli; Escherichia coli str. K-12 substr. MDS42; Thermus thermophilus; SYNTHETIC CONSTRUCT | 3 | X-RAY DIFFRACTION | 90.3 |
4V57 | RIBOSOME | 07/21/07 | Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin and neomycin. | Escherichia coli | 3.5 | X-RAY DIFFRACTION | 90.3 |
4V55 | RIBOSOME | 06/17/07 | Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin and ribosome recycling factor (RRF). | Escherichia coli; Thermus thermophilus | 4 | X-RAY DIFFRACTION | 90.3 |
7O1A | TRANSLATION | 03/29/21 | Cryo-EM structure of an Escherichia coli TnaC(R23F)-ribosome complex stalled in response to L-tryptophan | Escherichia coli (strain K12); Escherichia coli K-12; SYNTHETIC CONSTRUCT | 2.4 | ELECTRON MICROSCOPY | 90.3 |
7NAX | RIBOSOME | 01/25/21 | Complete Bacterial 30S ribosomal subunit assembly complex state I (Consensus Refinement) | Escherichia coli | 2.96 | ELECTRON MICROSCOPY | 90.3 |
7UNV | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.7 | ELECTRON MICROSCOPY | 100.0 |
6SPE | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 30s ribosome from a clinical isolate | Pseudomonas aeruginosa | 3.6 | ELECTRON MICROSCOPY | 100.0 |
7UNR | RIBOSOME | 04/11/22 | Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) | Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT | 2.9 | ELECTRON MICROSCOPY | 100.0 |
6SPG | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 70s ribosome from a clinical isolate | Pseudomonas aeruginosa | 3.34 | ELECTRON MICROSCOPY | 100.0 |
7OE1 | RIBOSOME | 04/30/21 | 30S ribosomal subunit from E. coli | Escherichia coli (strain K12); Escherichia coli str. K-12 substr. MG1655 | 3.05 | ELECTRON MICROSCOPY | 90.3 |
6SPF | RIBOSOME | 09/01/19 | Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate | Pseudomonas aeruginosa | 2.89 | ELECTRON MICROSCOPY | 100.0 |
7O1C | TRANSLATION | 03/29/21 | Cryo-EM structure of an Escherichia coli TnaC(R23F)-ribosome-RF2 complex stalled in response to L-tryptophan | Escherichia coli (strain K12); Escherichia coli K-12; SYNTHETIC CONSTRUCT | 2.6 | ELECTRON MICROSCOPY | 90.3 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
Nitrofurantoin | DB00698 | DrugBank | 5.1.4 | P0A7R5 | 30S ribosomal protein S10 | 90.3 | 103 | 2.8e-45 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 664 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001446 (528 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4264
Search term: rpsJ
Search term: 30S ribosomal protein S10
|
The primary structure of protein S10 from the small ribosomal subunit of Escherichia coli.
Yaguchi M, Roy C, Wittmann HG
FEBS Lett 1980 Nov 17;121(1):113-6
PubMed ID: 7007073
|
Structure of the Escherichia coli S10 ribosomal protein operon.
Zurawski G, Zurawski SM
Nucleic Acids Res 1985 Jun 25;13(12):4521-6
PubMed ID: 3892488
|
Direct interaction between two Escherichia coli transcription antitermination factors, NusB and ribosomal protein S10.
Mason SW, Li J, Greenblatt J
J Mol Biol 1992 Jan 5;223(1):55-66
PubMed ID: 1731086
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|