Pseudomonas aeruginosa PAO1, PA4264 (rpsJ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4264
Name
rpsJ
Replicon chromosome
Genomic location 4767343 - 4767654 (- strand)

Cross-References

RefSeq NP_252954.1
GI 15599460
Affymetrix PA4264_rpsJ_at
DNASU PaCD00007851
Entrez 881717
GenBank AAG07652.1
INSDC AAG07652.1
NCBI Locus Tag PA4264
protein_id(GenBank) gb|AAG07652.1|AE004842_1|gnl|PseudoCAP|PA4264
TIGR NTL03PA04265
UniParc UPI0000134BBD
UniProtKB Acc Q9HWD4
UniProtKB ID RS10_PSEAE
UniRef100 UniRef100_A6UZI7
UniRef50 UniRef50_P19460
UniRef90 UniRef90_B4RZN1

Product

Feature Type CDS
Coding Frame 1
Product Name
30S ribosomal protein S10
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Charge (pH 7) 4.71
Kyte-Doolittle Hydrophobicity Value -0.488
Molecular Weight (kDa) 11.8
Isoelectric Point (pI) 10.47

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7N1P RIBOSOME 05/28/21 Elongating 70S ribosome complex in a classical pre-translocation (PRE-C) conformation Escherichia coli (strain K12); Escherichia coli K-12; SYNTHETIC CONSTRUCT 2.33 ELECTRON MICROSCOPY 90.3
4V56 RIBOSOME 07/21/07 Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. Escherichia coli 3.93 X-RAY DIFFRACTION 90.3
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPC RIBOSOME 09/01/19 Pseudomonas aeruginosa 30s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.95 ELECTRON MICROSCOPY 100.0
4V5B RIBOSOME 11/22/07 Structure of PDF binding helix in complex with the ribosome. ESCHERICHIA COLI; SYNTHETIC CONSTRUCT 3.74 X-RAY DIFFRACTION 90.3
4V6C RIBOSOME 06/27/09 Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting Escherichia coli; Escherichia coli K-12 3.19 X-RAY DIFFRACTION 90.3
7NAV RIBOSOME 01/25/21 Bacterial 30S ribosomal subunit assembly complex state D (Consensus refinement) Escherichia coli (strain K12) 4.8 ELECTRON MICROSCOPY 90.3
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
7NWT RIBOSOME 03/17/21 Initiated 70S ribosome in complex with 2A protein from encephalomyocarditis virus (EMCV) Escherichia coli; Mengo encephalomyocarditis virus; SYNTHETIC CONSTRUCT 2.66 ELECTRON MICROSCOPY 90.3
7OE0 RIBOSOME 04/30/21 E. coli pre-30S delta rbfA ribosomal subunit class F Escherichia coli BW25113 2.69 ELECTRON MICROSCOPY 90.3
7O19 TRANSLATION 03/29/21 Cryo-EM structure of an Escherichia coli TnaC-ribosome complex stalled in response to L-tryptophan Escherichia coli K-12; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 90.3
4WOI RIBOSOME 10/15/14 4,5-linked aminoglycoside antibiotics regulate the bacterial ribosome by targeting dynamic conformational processes within intersubunit bridge B2 Escherichia coli; Escherichia coli str. K-12 substr. MDS42; Thermus thermophilus; SYNTHETIC CONSTRUCT 3 X-RAY DIFFRACTION 90.3
4V57 RIBOSOME 07/21/07 Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin and neomycin. Escherichia coli 3.5 X-RAY DIFFRACTION 90.3
4V55 RIBOSOME 06/17/07 Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin and ribosome recycling factor (RRF). Escherichia coli; Thermus thermophilus 4 X-RAY DIFFRACTION 90.3
7O1A TRANSLATION 03/29/21 Cryo-EM structure of an Escherichia coli TnaC(R23F)-ribosome complex stalled in response to L-tryptophan Escherichia coli (strain K12); Escherichia coli K-12; SYNTHETIC CONSTRUCT 2.4 ELECTRON MICROSCOPY 90.3
7NAX RIBOSOME 01/25/21 Complete Bacterial 30S ribosomal subunit assembly complex state I (Consensus Refinement) Escherichia coli 2.96 ELECTRON MICROSCOPY 90.3
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
6SPE RIBOSOME 09/01/19 Pseudomonas aeruginosa 30s ribosome from a clinical isolate Pseudomonas aeruginosa 3.6 ELECTRON MICROSCOPY 100.0
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0
7OE1 RIBOSOME 04/30/21 30S ribosomal subunit from E. coli Escherichia coli (strain K12); Escherichia coli str. K-12 substr. MG1655 3.05 ELECTRON MICROSCOPY 90.3
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 100.0
7O1C TRANSLATION 03/29/21 Cryo-EM structure of an Escherichia coli TnaC(R23F)-ribosome-RF2 complex stalled in response to L-tryptophan Escherichia coli (strain K12); Escherichia coli K-12; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 90.3

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Nitrofurantoin DB00698 DrugBank 5.1.4 P0A7R5 30S ribosomal protein S10 90.3 103 2.8e-45

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 664 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001446 (528 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4264
Search term: rpsJ

Human Homologs

References

The primary structure of protein S10 from the small ribosomal subunit of Escherichia coli.
Yaguchi M, Roy C, Wittmann HG
FEBS Lett 1980 Nov 17;121(1):113-6
PubMed ID: 7007073
Structure of the Escherichia coli S10 ribosomal protein operon.
Zurawski G, Zurawski SM
Nucleic Acids Res 1985 Jun 25;13(12):4521-6
PubMed ID: 3892488
Direct interaction between two Escherichia coli transcription antitermination factors, NusB and ribosomal protein S10.
Mason SW, Li J, Greenblatt J
J Mol Biol 1992 Jan 5;223(1):55-66
PubMed ID: 1731086
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142