Pseudomonas aeruginosa PAO1, PA4267 (rpsG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4267
Name
rpsG
Replicon chromosome
Genomic location 4771186 - 4771656 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_252957.1
GI 15599463
Affymetrix PA4267_rpsG_at
Entrez 881745
GenBank AAG07655.1
INSDC AAG07655.1
NCBI Locus Tag PA4267
protein_id(GenBank) gb|AAG07655.1|AE004842_4|gnl|PseudoCAP|PA4267
TIGR NTL03PA04268
UniParc UPI0000135112
UniProtKB Acc Q9HWD1
UniProtKB ID RS7_PSEAE
UniRef100 UniRef100_A6UZI4
UniRef50 UniRef50_P9WH28
UniRef90 UniRef90_Q889X5

Product

Feature Type CDS
Coding Frame 1
Product Name
30S ribosomal protein S7
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (17.50 kDa, PMID:21751344)
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 14.71
Kyte-Doolittle Hydrophobicity Value -0.485
Molecular Weight (kDa) 17.5
Isoelectric Point (pI) 10.92

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6SPE RIBOSOME 09/01/19 Pseudomonas aeruginosa 30s ribosome from a clinical isolate Pseudomonas aeruginosa 3.6 ELECTRON MICROSCOPY 100.0
6SPC RIBOSOME 09/01/19 Pseudomonas aeruginosa 30s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.95 ELECTRON MICROSCOPY 100.0
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 646 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001444 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4267
Search term: rpsG

Human Homologs

Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein S7 [Source:HGNC Symbol;Acc:HGNC:14499]
E-value: 1.3e-14
Percent Identity: 31.6
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein S7 [Source:HGNC Symbol;Acc:HGNC:14499]
E-value: 1.3e-14
Percent Identity: 31.6
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein S7 [Source:HGNC Symbol;Acc:HGNC:14499]
E-value: 1.3e-14
Percent Identity: 31.6
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein S7 [Source:HGNC Symbol;Acc:HGNC:14499]
E-value: 1.3e-14
Percent Identity: 31.6

References

DNA sequences from the str operon of Escherichia coli.
Post LE, Nomura M
J Biol Chem 1980 May 25;255(10):4660-6
PubMed ID: 6989816
Comparison of the complete sequence of the str operon in Salmonella typhimurium and Escherichia coli.
Johanson U, Hughes D
Gene 1992 Oct 12;120(1):93-8
PubMed ID: 1398129
Post-transcriptional regulation of the str operon in Escherichia coli. Ribosomal protein S7 inhibits coupled translation of S7 but not its independent translation.
Saito K, Mattheakis LC, Nomura M
J Mol Biol 1994 Jan 7;235(1):111-24
PubMed ID: 7507167
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344