Pseudomonas aeruginosa PAO1, PA4268 (rpsL)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4268
Name
rpsL
Synonym: str
Replicon chromosome
Genomic location 4771756 - 4772127 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_252958.1
GI 15599464
Affymetrix PA4268_rpsL_at
DNASU PaCD00007852
Entrez 881709
GenBank AAG07656.1
INSDC AAG07656.1
NCBI Locus Tag PA4268
protein_id(GenBank) gb|AAG07656.1|AE004842_5|gnl|PseudoCAP|PA4268
TIGR NTL03PA04269
UniParc UPI0000134C69
UniProtKB Acc Q9HWD0
UniProtKB ID RS12_PSEAE
UniRef100 UniRef100_A6UZI3
UniRef50 UniRef50_P61941
UniRef90 UniRef90_A6W397

Product

Feature Type CDS
Coding Frame 1
Product Name
30S ribosomal protein S12
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 20.61
Kyte-Doolittle Hydrophobicity Value -0.818
Molecular Weight (kDa) 13.8
Isoelectric Point (pI) 11.60

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
6SPC RIBOSOME 09/01/19 Pseudomonas aeruginosa 30s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.95 ELECTRON MICROSCOPY 100.0
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0
6SPE RIBOSOME 09/01/19 Pseudomonas aeruginosa 30s ribosome from a clinical isolate Pseudomonas aeruginosa 3.6 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 660 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001443 (531 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4268
Search term: rpsL

Human Homologs

Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein S12 [Source:HGNC Symbol;Acc:HGNC:10380]
E-value: 9.5e-21
Percent Identity: 49.5
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein S12 [Source:HGNC Symbol;Acc:HGNC:10380]
E-value: 9.5e-21
Percent Identity: 49.5
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein S12 [Source:HGNC Symbol;Acc:HGNC:10380]
E-value: 9.5e-21
Percent Identity: 49.5
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein S12 [Source:HGNC Symbol;Acc:HGNC:10380]
E-value: 9.5e-21
Percent Identity: 49.5
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein S12 [Source:HGNC Symbol;Acc:HGNC:10380]
E-value: 9.5e-21
Percent Identity: 49.5
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein S12 [Source:HGNC Symbol;Acc:HGNC:10380]
E-value: 9.5e-21
Percent Identity: 49.5

References

The nucleotide sequence of rpsL and its flanking regions in Salmonella typhimurium.
Hughes D, Buckingham RH
Gene 1991 Jul 31;104(1):123-4
PubMed ID: 1916272
Mutant sequences in the rpsL gene of Escherichia coli B/r: mechanistic implications for spontaneous and ultraviolet light mutagenesis.
Timms AR, Steingrimsdottir H, Lehmann AR, Bridges BA
Mol Gen Genet 1992 Mar;232(1):89-96
PubMed ID: 1552908
Modelling in Escherichia coli of mutations in mitoribosomal protein S12: novel mutant phenotypes of rpsL.
Toivonen JM, Boocock MR, Jacobs HT
Mol Microbiol 1999 Mar;31(6):1735-46
PubMed ID: 10209746
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142