Pseudomonas aeruginosa PAO1, PA4272 (rplJ)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA4272
Name	rplJ
Replicon	chromosome
Genomic location	4781286 - 4781786 (- strand)
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_252962.1
GI	15599468
Affymetrix	PA4272_rplJ_at
DNASU	PaCD00007854
Entrez	881702
GenBank	AAG07660.1
INSDC	AAG07660.1
NCBI Locus Tag	PA4272
protein_id(GenBank)	gb|AAG07660.1|AE004843_2|gnl|PseudoCAP|PA4272
TIGR	NTL03PA04273
UniParc	UPI0000133B45
UniProtKB Acc	Q9HWC7
UniProtKB ID	RL10_PSEAE
UniRef100	UniRef100_A6UZH9
UniRef50	UniRef50_P44350
UniRef90	UniRef90_Q889Y0

Product

Feature Type	CDS
Coding Frame	1
Product Name	50S ribosomal protein L10 Product Name Confidence: Class 2
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected by two-dimensional gel electrophoresis and protein mass mapping(PMID:21863142).
Charge (pH 7)	2.23
Kyte-Doolittle Hydrophobicity Value	0.096
Molecular Weight (kDa)	17.6
Isoelectric Point (pI)	9.53

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
7UNU	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
7UNV	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.7	ELECTRON MICROSCOPY	100.0
7UNR	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	100.0
7UNW	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.6	ELECTRON MICROSCOPY	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 576 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001439 (534 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA4272 Search term: rplJ Search term: 50S ribosomal protein L10

Human Homologs

References

The primary structure of protein L10 from Escherichia coli ribosomes. Dovgas NV, Vinokurov LM, Velmoga IS, Alakhov YB, Ovchinnikov YA FEBS Lett 1976 Aug 1;67(1):58-61 PubMed ID: 782920

Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in Escherichia coli. Post LE, Strycharz GD, Nomura M, Lewis H, Dennis PP Proc Natl Acad Sci U S A 1979 Apr;76(4):1697-701 PubMed ID: 377281

Feedback regulation of the rplJL-rpoBC ribosomal protein operon of Escherichia coli requires a region of mRNA secondary structure. Climie SC, Friesen JD J Mol Biol 1987 Dec 5;198(3):371-81 PubMed ID: 2448482

Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient. Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB Front Microbiol 2011;2:162 PubMed ID: 21863142