Pseudomonas aeruginosa PAO1, PA4275 (nusG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4275
Name
nusG
Replicon chromosome
Genomic location 4783228 - 4783761 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252965.1
GI 15599471
Affymetrix PA4275_nusG_at
Entrez 881693
GenBank AAG07663.1
INSDC AAG07663.1
NCBI Locus Tag PA4275
protein_id(GenBank) gb|AAG07663.1|AE004843_5|gnl|PseudoCAP|PA4275
TIGR NTL03PA04276
UniParc UPI0000130A84
UniProtKB Acc Q9HWC4
UniProtKB ID NUSG_PSEAE
UniRef100 UniRef100_Q9HWC4
UniRef50 UniRef50_Q9HWC4
UniRef90 UniRef90_Q9HWC4

Product

Feature Type CDS
Coding Frame 1
Product Name
transcription antitermination protein NusG
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Charge (pH 7) -2.28
Kyte-Doolittle Hydrophobicity Value -0.384
Molecular Weight (kDa) 20.1
Isoelectric Point (pI) 5.81

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 589 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001436 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4275
Search term: nusG

Human Homologs

References

Elongation factor NusG interacts with termination factor rho to regulate termination and antitermination of transcription.
Li J, Mason SW, Greenblatt J
Genes Dev 1993 Jan;7(1):161-72
PubMed ID: 8422985
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142