Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4277
|
Name |
tufB
|
Replicon | chromosome |
Genomic location | 4784316 - 4785509 (- strand) |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_252967.1 |
GI | 15599473 |
Entrez | 881697 |
GenBank | AAG07665.1 |
NCBI Locus Tag | PA4277 |
protein_id(GenBank) | gb|AAG07665.1|AE004843_7|gnl|PseudoCAP|PA4277 |
TIGR | NTL03PA04278 |
UniProtKB Acc | P09591 |
UniProtKB ID | EFTU_PSEAE |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
elongation factor Tu
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (43.37 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
|
Charge (pH 7) | -12.75 |
Kyte-Doolittle Hydrophobicity Value | -0.152 |
Molecular Weight (kDa) | 43.4 |
Isoelectric Point (pI) | 5.03 |
Individual Mappings | |
Additional evidence for subcellular localization |
Detetected in a periplasmic protein preparation by spheroplasting by osmotic shock.(PMID:19333994)
|
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
4ZV4 | TRANSLATION | 05/18/15 | Structure of Tse6 in complex with EF-Tu | Pseudomonas aeruginosa; Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) | 3.504 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001434 (870 members) |
Putative Inparalogs |
1 predicted inparalogs elongation factor Tu (PA4265) 100.0% identity |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4277
Search term: tufB
Search term: elongation factor Tu
|
Ensembl
110, assembly
GRCh38.p14
|
Tu translation elongation factor, mitochondrial [Source:HGNC Symbol;Acc:HGNC:12420]
E-value:
6.4e-135
Percent Identity:
55.9
|
The nucleotide sequence of tufB and four nearby tRNA structural genes of Escherichia coli.
An G, Friesen JD
Gene 1980 Dec;12(1-2):33-9
PubMed ID: 7011904
|
The amino acid sequence of elongation factor Tu of Escherichia coli. The complete sequence.
Laursen RA, L'Italien JJ, Nagarkatti S, Miller DL
J Biol Chem 1981 Aug 10;256(15):8102-9
PubMed ID: 7021545
|
The structure of the Escherichia coli EF-Tu.EF-Ts complex at 2.5 A resolution.
Kawashima T, Berthet-Colominas C, Wulff M, Cusack S, Leberman R
Nature 1996 Feb 8;379(6565):511-8
PubMed ID: 8596629
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|