Pseudomonas aeruginosa PAO1, PA4284 (recB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4284
Name
recB
Synonym: rorA
Replicon chromosome
Genomic location 4801461 - 4805198 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252974.1
GI 15599480
Affymetrix PA4284_recB_at
Entrez 881653
GenBank AAG07672.1
INSDC AAG07672.1
NCBI Locus Tag PA4284
protein_id(GenBank) gb|AAG07672.1|AE004844_4|gnl|PseudoCAP|PA4284
TIGR NTL03PA04285
UniParc UPI00000C5C95
UniProtKB Acc Q9HWB6
UniProtKB ID Q9HWB6_PSEAE
UniRef100 UniRef100_Q9HWB6
UniRef50 UniRef50_Q9HWB6
UniRef90 UniRef90_Q9HWB6

Product

Feature Type CDS
Coding Frame 1
Product Name
exodeoxyribonuclease V beta chain
Product Name Confidence: Class 2
Synonyms exonuclease V 135 kD polypeptide
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -30.00
Kyte-Doolittle Hydrophobicity Value -0.362
Molecular Weight (kDa) 139.4
Isoelectric Point (pI) 5.45

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 537 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003887 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4284
Search term: recB

Human Homologs

References

Fine structure of the recB and recC gene region of Escherichia coli.
Sasaki M, Fujiyoshi T, Shimada K, Takagi Y
Biochem Biophys Res Commun 1982 Nov 30;109(2):414-22
PubMed ID: 6295393
Properties of the recB and recC gene products of Escherichia coli.
Umeno M, Sasaki M, Anai M, Takagi Y
Biochem Biophys Res Commun 1983 Nov 15;116(3):1144-50
PubMed ID: 6316968
Reconstitution of RecBC DNase activity from purified Escherichia coli RecB and RecC proteins.
Hickson ID, Robson CN, Atkinson KE, Hutton L, Emmerson PT
J Biol Chem 1985 Jan 25;260(2):1224-9
PubMed ID: 3155726